兵庫県立大学 ピコバイオロジー研究所

電話でのお問い合わせはTEL.0791-58-0191

〒678-1297 兵庫県赤穂郡上郡町光都3-2-1





研究業績Study achievements


2000

  • X-ray structure and the reaction mechanism of bovine heart cytochrome c oxidase. Yoshikawa S, Shinzawa-Itoh K, Tsukihara T. J Inorg Biochem. 82(1-4), 1-7 (2000)
  • X-ray structure of azide-bound fully oxidized cytochrome c oxidase from bovine heart at 2.9 A resolution. Fei MJ, Yamashita E, Inoue N, Yao M, Yamaguchi H, Tsukihara T, Shinzawa-Itoh K, Nakashima R, Yoshikawa S. Acta Crystallogr D Biol Crystallogr. 56(Pt 5), 529-35 (2000)

1999

  • Examination of the reaction of fully reduced cytochrome oxidase with hydrogen peroxide by flow-flash spectroscopy. Zaslavsky D, Smirnova IA, Brzezinski P, Shinzawa-Itoh K, Yoshikawa S, Gennis RB. Biochemistry. 38(48), 16016-23 (1999)
  • Quantitative reevaluation of the redox active sites of crystalline bovine heart cytochrome c oxidase. Mochizuki M, Aoyama H, Shinzawa-Itoh K, Usui T, Tsukihara T, Yoshikawa S. J Biol Chem. 274(47), 33403-11 (1999)
  • Direct metal analyses of Mn2+-dependent and -independent protein phosphatase 2A from human erythrocytes detect zinc and iron only in the Mn2+-independent one. Nishito Y, Usui H, Shinzawa-Itoh K, Inoue R, Tanabe O, Nagase T, Murakami T, Takeda M. FEBS Lett. 447(1), 29-33 (1999)
  • Structure analysis of bovine heart cytochrome c oxidase at 2.8 A resolution. Tomizaki T, Yamashita E, Yamaguchi H, Aoyama H, Tsukihara T, Shinzawa-Itoh K, Nakashima R, Yaono R, Yoshikawa S. Acta Crystallogr D Biol Crystallogr. 55(Pt 1), 31-45 (1999)

1998

  • Crystals of bovine heart ubiquinol-cytochrome c reductase diffracting X-rays up to 2.8 A resolution at 276 K. Murakami S, Shinzawa-Itoh K, Yoshikawa S. Acta Crystallogr D Biol Crystallogr. 54(Pt 1), 146-7 (1998)
  • Redox-coupled crystal structural changes in bovine heart cytochrome c oxidase. Yoshikawa S, Shinzawa-Itoh K, Nakashima R, Yaono R, Yamashita E, Inoue N, Yao M, Fei MJ, Libeu CP, Mizushima T, Yamaguchi H, Tomizaki T, Tsukihara T. Science. 280(5370), 1723-9 (1998)
  • Crystal structure of bovine heart cytochrome c oxidase at 2.8 A resolution. Yoshikawa S, Shinzawa-Itoh K, Tsukihara T. J Bioenerg Biomembr. 30(1), 7-14 (1998)

1997

  • ATP and ADP bind to cytochrome c oxidase and regulate its activity. Napiwotzki J, Shinzawa-Itoh K, Yoshikawa S, Kadenbach B. Biol Chem. 378(9), 1013-21 (1997)

1996

  • Crystal structure of fully oxidized cytochrome c-oxidase from the bovine heart at 2.8 A resolution]. Yoshikawa S, Tsukhihara T, Shinzawa-Itoh K. Biokhimiia. 61(11), 1931-40 (1997)
  • Resonance Raman/absorption characterization of the oxo intermediates of cytochrome c oxidase generated in its reaction with hydrogen peroxide: pH and H2O2 concentration dependence. Proshlyakov DA, Ogura T, Shinzawa-Itoh K, Yoshikawa S, Kitagawa T. Biochemistry. 35(26), 8580-6 (1996)
  • The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A. Tsukihara T, Aoyama H, Yamashita E, Tomizaki T, Yamaguchi H, Shinzawa-Itoh K, Nakashima R, Yaono R, Yoshikawa S. Science. 272(5265), 1136-44 (1996)
  • Microcirculating system for simultaneous determination of Raman and absorption spectra of enzymatic reaction intermediates and its application to the reaction of cytochrome c oxidase with hydrogen peroxide. Proshlyakov DA, Ogura T, Shinzawa-Itoh K, Yoshikawa S, Kitagawa T. Biochemistry. 35(1), 76-82 (1996)

1995

  • Structures of metal sites of oxidized bovine heart cytochrome c oxidase at 2.8 A. Tsukihara T, Aoyama H, Yamashita E, Tomizaki T, Yamaguchi H, Shinzawa-Itoh K, Nakashima R, Yaono R, Yoshikawa S. Science. 269(5227), 1069-74 (1995)
  • Effects of ethyleneglycol chain length of dodecyl polyethyleneglycol monoether on the crystallization of bovine heart cytochrome c oxidase. Shinzawa-Itoh K, Ueda H, Yoshikawa S, Aoyama H, Yamashita E, Tsukihara T. J Mol Biol. 246(5), 572-5 (1995)

1994

  • New crystal forms and preliminary X-ray diffraction studies of mitochondrial cytochrome bc1 complex from bovine heart. Kawamoto M, Kubota T, Matsunaga T, Fukuyama K, Matsubara H, Shinzawa-Itoh K, Yoshikawa S. J Mol Biol. 244(2), 238-41 (1994)
  • Selective resonance Raman observation of the "607 nm" form generated in the reaction of oxidized cytochrome c oxidase with hydrogen peroxide. Proshlyakov DA, Ogura T, Shinzawa-Itoh K, Yoshikawa S, Appelman EH, Kitagawa T. J Biol Chem. 269(47), 29385-8 (1994)

1993

  • Time-resolved resonance Raman study on the binding of carbon monoxide to recombinant human myoglobin and its distal histidine mutants. Sakan Y, Ogura T, Kitagawa T, Fraunfelter FA, Mattera R, Ikeda-Saito M. Biochemistry. 32(22), 5815-24 (1993)
  • Resonance Raman studies on the CuA site of cytochrome c oxidase using a multichannel scanning Raman spectrometer with a CCD detector. Takahashi S, Ogura T, Shinzawa-Itoh K, Yoshikawa S, Kitagawa T. Biochemistry. 32(14), 3664-70 (1993)

1992

  • Single crystals of bovine heart cytochrome c oxidase at fully oxidized resting, fully reduced and CO-bound fully reduced states are isomorphous with each other. Shinzawa-Itoh K, Yamashita H, Yoshikawa S, Fukumoto Y, Abe T, Tsukihara T. J Mol Biol. 228(3), 987-90 (1992)
  • A high-resolution solid-state 13C-NMR study on crystalline bovine heart cytochrome-c oxidase and lysozyme. Dynamic behavior of protein and detergent in the complex. Tuzi S, Shinzawa-Itoh K, Erata T, Naito A, Yoshikawa S, Saitô H. Eur J Biochem. 208(3), 713-20 (1992)

1991

  • Crystallization and preliminary X-ray crystallographic studies of bovine heart mitochondrial cytochrome bc1 complex. Kubota T, Kawamoto M, Fukuyama K, Shinzawa-Itoh K, Yoshikawa S, Matsubara H. J Mol Biol. 221(2), 379-82 (1991)

1990

  • Observation of the Fe4+ = O stretching Raman band for cytochrome oxidase compound B at ambient temperature. Ogura T, Takahashi S, Shinzawa-Itoh K, Yoshikawa S, Kitagawa T. J Biol Chem. 265(25), 14721-3 (1991)


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タンパク質結晶成長機構兵庫県立大学 ピコバイオロジー研究所

〒678-1297
兵庫県赤穂郡上郡町
光都3-2-1
TEL 0791-58-0191
FAX 0791-58-0489