Effects of proton motive force on the structure and dynamics of bovine
cytochrome C oxidase in phospholipid vesicles. Nomura T, Yanagisawa S,
Shinzawa-Itoh K, Yoshikawa S, Ogura T. Biochemistry. 53(40), 6382-91 (2014）
Determination of damage-free crystal structure of an X-ray-sensitive protein
using an XFEL. Hirata K, Shinzawa-Itoh K, Yano N, Takemura S, Kato K, Hatanaka
M, Muramoto K, Kawahara T, Tsukihara T, Yamashita E, Tono K, Ueno G, Hikima
T, Murakami H, Inubushi Y, Yabashi M, Ishikawa T, Yamamoto M, Ogura T,
Sugimoto H, Shen JR, Yoshikawa S, Ago H. Nat Methods. 11(7), 734-6 (2014)
Three-dimensional structure of bovine heart NADH: ubiquinone oxidoreductase
(complex I) by electron microscopy of a single negatively stained two-dimensional
crystal. Shimada S, Shinzawa-Itoh K, Amano S, Akira Y, Miyazawa A, Tsukihara
T, Tani K, Gerle C, Yoshikawa S. Microscopy (Oxf). 63(2), 167-74 (2014)
2013
Two-dimensional crystallization of intact F-ATP synthase isolated from
bovine heart mitochondria. Maeda S, Shinzawa-Itoh K, Mieda K, Yamamoto
M, Nakashima Y, Ogasawara Y, Jiko C, Tani K, Miyazawa A, Gerle C, Yoshikawa
S. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69(Pt 12), 1368-70
(2013)
Effective pumping proton collection facilitated by a copper site (CuB)
of bovine heart cytochrome c oxidase, revealed by a newly developed time-resolved
infrared system. Kubo M, Nakashima S, Yamaguchi S, Ogura T, Mochizuki M,
Kang J, Tateno M, Shinzawa-Itoh K, Kato K, Yoshikawa S. J Biol Chem. 288(42),
30259-69 (2013)
Resonance Raman spectral properties of FMN of bovine heart NADH: ubiquinone
oxidoreductase suggesting a mechanism for the prevention of spontaneous
production of reactive oxygen species. Hikita M, Shinzawa-Itoh K, Moriyama
M, Ogura T, Kihira K, Yoshikawa S. Biochemistry. 52(1), 98-104 (2013)
2012
Reaction mechanism of mammalian mitochondrial cytochrome c oxidase. Yoshikawa
S, Muramoto K, Shinzawa-Itoh K. Adv Exp Med Biol. 748, 215-36 (2012)
EPR detection of two protein-associated ubiquinone components (SQ(Nf) and
SQ(Ns)) in the membrane in situ and in proteoliposomes of isolated bovine
heart complex I. Ohnishi T, Ohnishi ST, Shinzawa-Itoh K, Yoshikawa S, Weber
RT. Biochim Biophys Acta. 1817(10), 1803-9 (2012)
Two tyrosyl radicals stabilize high oxidation states in cytochrome C oxidase
for efficient energy conservation and proton translocation. Yu MA, Egawa
T, Shinzawa-Itoh K, Yoshikawa S, Guallar V, Yeh SR, Rousseau DL, Gerfen
GJ. J Am Chem Soc. 134(10), 4753-61 (2012)
Structural studies on bovine heart cytochrome c oxidase. Yoshikawa S, Muramoto
K, Shinzawa-Itoh K, Mochizuki M. Biochim Biophys Acta. 1817(4), 579-89
(2012)
2011
Distinguishing between Cl- and O2(2-) as the bridging element between Fe3+
and Cu2+ in resting-oxidized cytochrome c oxidase. Suga M, Yano N, Muramoto K,
Shinzawa-Itoh K, Maeda T, Yamashita E, Tsukihara T, Yoshikawa S. Acta
Crystallogr D Biol Crystallogr. 67(Pt 8), 742-4 (2011)
NMR basis for interprotein electron transfer gating between cytochrome c and
cytochrome c oxidase. Sakamoto K, Kamiya M, Imai M, Shinzawa-Itoh K, Uchida T,
Kawano K, Yoshikawa S, Ishimori K. Proc Natl Acad Sci U S A. 108(30), 12271-6
(2011)
The O(2) reduction and proton pumping gate mechanism of bovine heart
cytochrome c oxidase. Yoshikawa S, Muramoto K, Shinzawa-Itoh K. Biochim Biophys
Acta. 1807(10), 1279-86 (2011)
Proton-pumping mechanism of cytochrome C oxidase. Yoshikawa S, Muramoto K,
Shinzawa-Itoh K. Annu Rev Biophys. 40, 205-23 (2011)
2010
New insights into the superoxide generation sites in bovine heart
NADH-ubiquinone oxidoreductase (Complex I): the significance of
protein-associated ubiquinone and the dynamic shifting of generation sites
between semiflavin and semiquinone radicals. Ohnishi ST, Shinzawa-Itoh K, Ohta
K, Yoshikawa S, Ohnishi T. Biochim Biophys Acta. 1797(12), 1901-9 (2010)
Bovine cytochrome c oxidase structures enable O2 reduction with minimization
of reactive oxygens and provide a proton-pumping gate. Muramoto K, Ohta K,
Shinzawa-Itoh K, Kanda K, Taniguchi M, Nabekura H, Yamashita E, Tsukihara T,
Yoshikawa S. Proc Natl Acad Sci U S A. 107(17), 7740-5 (2010)
Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane
protein. Katayama Y, Shimokata K, Suematsu M, Ogura T, Tsukihara T, Yoshikawa S,
Shimada H. J Bioenerg Biomembr. 42(3), 235-40 (2010)
A resonance Raman band assignable to the O-O stretching mode in the resting
oxidized state of bovine heart cytochrome c oxidase. Sakaguchi M, Shinzawa-Itoh
K, Yoshikawa S, Ogura T. J Bioenerg Biomembr. 42(3), 241-3 (2010)
X-ray structure of the NO-bound Cu(B) in bovine cytochrome c oxidase. Ohta
K, Muramoto K, Shinzawa-Itoh K, Yamashita E, Yoshikawa S, Tsukihara T. Acta
Crystallogr Sect F Struct Biol Cryst Commun. 66(Pt 3), 251-3. (2010)
Bovine heart NADH-ubiquinone oxidoreductase contains one molecule of
ubiquinone with ten isoprene units as one of the cofactors. Shinzawa-Itoh K,
Seiyama J, Terada H, Nakatsubo R, Naoki K, Nakashima Y, Yoshikawa S.
Biochemistry. 49(3), 487-92 (2010)