兵庫県立大学 ピコバイオロジー研究所

電話でのお問い合わせはTEL.0791-58-0191

〒678-1297 兵庫県赤穂郡上郡町光都3-2-1





研究業績Study achievements

2009

  • Lipid-protein interactions in bovine heart cytochrome c oxidase and other membrane proteins. Yoshikawa S. Tanpakushitsu Kakusan Koso. 54(14), 1827-36 (2009)
  • A peroxide bridge between Fe and Cu ions in the O2 reduction site of fully oxidized cytochrome c oxidase could suppress the proton pump. Aoyama H, Muramoto K, Shinzawa-Itoh K, Hirata K, Yamashita E, Tsukihara T, Ogura T, Yoshikawa S. Proc Natl Acad Sci U S A. 106(7), 2165-9 (2009)

2008

  • Functional role of coenzyme Q in the energy coupling of NADH-CoQ oxidoreductase (Complex I): stabilization of the semiquinone state with the application of inside-positive membrane potential to proteoliposomes. Ohnishi T, Ohnishi ST, Shinzawa-Ito K, Yoshikawa S. Biofactors. 32(1-4), 13-22 (2008)
  • Red-excitation resonance Raman analysis of the nu(Fe=O) mode of ferryl-oxo hemoproteins. Ikemura K, Mukai M, Shimada H, Tsukihara T, Yamaguchi S, Shinzawa-Itoh K, Yoshikawa S, Ogura T. J Am Chem Soc. 130(44), 14384-5 (2008)

2007

  • A histidine residue acting as a controlling site for dioxygen reduction and proton pumping by cytochrome c oxidase. Muramoto K, Hirata K, Shinzawa-Itoh K, Yoko-o S, Yamashita E, Aoyama H, Tsukihara T, Yoshikawa S. Proc Natl Acad Sci U S A. 104(19), 7881-6 (2007)
  • Resonance Raman spectra of the FMN of the bovine heart NADH: ubiquinone oxidoreductase, the largest membrane protein in the mitochondrial respiratory system. Sugiyama H, Nakatsubo R, Yamaguchi S, Ogura T, Shinzawa-Itoh K, Yoshikawa S. J Bioenerg Biomembr. 39(2), 145-8 (2007)
  • The proton pumping pathway of bovine heart cytochrome c oxidase. Shimokata K, Katayama Y, Murayama H, Suematsu M, Tsukihara T, Muramoto K, Aoyama H, Yoshikawa S, Shimada H. Proc Natl Acad Sci U S A. 104(10), 4200-5 (2007)
  • Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase. Shinzawa-Itoh K, Aoyama H, Muramoto K, Terada H, Kurauchi T, Tadehara Y, Yamasaki A, Sugimura T, Kurono S, Tsujimoto K, Mizushima T, Yamashita E, Tsukihara T, Yoshikawa S. EMBO J. 26(6), 1713-25 (2007)

2006

  • Proton pumping mechanism of bovine heart cytochrome c oxidase. Yoshikawa S, Muramoto K, Shinzawa-Itoh K, Aoyama H, Tsukihara T, Shimokata K, Katayama Y, Shimada H. Biochim Biophys Acta. 1757(9-10), 1110-6 (2006)
  • Reaction mechanism of bovine heart cytochrome c oxidase. Yoshikawa S, Muramoto K, Shinzawa-Itoh K, Aoyama H, Tsukihara T, Ogura T, Shimokata K, Katayama Y, Shimada H. Biochim Biophys Acta. 1757(5-6), 395-400 (2006)
  • Absolute configuration of the hydroxyfarnesylethyl group of haem A, determined by X-ray structural analysis of bovine heart cytochrome c oxidase using methods applicable at 2.8 Angstrom resolution. Yamashita E, Aoyama H, Yao M, Muramoto K, Shinzawa-Itoh K, Yoshikawa S, Tsukihara T. Acta Crystallogr D Biol Crystallogr. 61(Pt 10), 1373-7 (2006)

2005

  • Probing the oxygen activation reaction in intact whole mitochondria through analysis of molecular vibrations. Takahashi T, Kuroiwa S, Ogura T, Yoshikawa S. J Am Chem Soc. 127(28), 9970-1 (2005)

2004

  • The intrinsic stability of the second intermediate following the dioxygen-bound form in the O2 reduction by cytochrome c oxidase. Oda K, Ogura T, Appelman EH, Yoshikawa S. FEBS Lett. ;570(1-3), 161-5 (2004)
  • Scaling of one-shot oscillation images with a reference data set. Hirata K, Yamashita E, Aoyama H, Muramoto K, Yoshikawa S, Tsukihara T. J Synchrotron Radiat. 11(Pt 1), 60-3 (2004)

2003

  • Effect of pH on the steady state kinetics of bovine heart NADH: coenzyme Q oxidoreductase. Hano N, Nakashima Y, Shinzawa-Itoh K, Terada H, Yoshikawa S. J Bioenerg Biomembr. 35(5), 419-25 (2003)
  • The low-spin heme of cytochrome c oxidase as the driving element of the proton-pumping process. Tsukihara T, Shimokata K, Katayama Y, Shimada H, Muramoto K, Aoyama H, Mochizuki M, Shinzawa-Itoh K, Yamashita E, Yao M, Ishimura Y, Yoshikawa S. Proc Natl Acad Sci U S A. 100(26):15304-9 (2003)
  • A cytochrome c oxidase proton pumping mechanism that excludes the O2 reduction site. Yoshikawa S. FEBS Lett. 555(1), 8-12 (2003)
  • Effect of the side chain structure of coenzyme Q on the steady state kinetics of bovine heart NADH: coenzyme Q oxidoreductase. Hano N, Nakashima Y, Shinzawa-Itoh K, Yoshikawa S. J Bioenerg Biomembr. 35(3), 257-65 (2003)
  • FTIR detection of protonation/deprotonation of key carboxyl side chains caused by redox change of the Cu(A)-heme a moiety and ligand dissociation from the heme a3-Cu(B) center of bovine heart cytochrome c oxidase. Okuno D, Iwase T, Shinzawa-Itoh K, Yoshikawa S, Kitagawa T. J Am Chem Soc. 125(24), 7209-18 (2003)

2002

  • Cytochrome c oxidase. Yoshikawa S. Adv Protein Chem. 60, 341-95 (2002)
  • The second coenzyme Q1 binding site of bovine heart NADH: coenzyme Q oxidoreductase. Nakashima Y, Shinzawa-Itoh K, Watanabe K, Naoki K, Hano N, Yoshikawa S. J Bioenerg Biomembr. 34(2), 89-94 (2002)
  • Steady-state kinetics of NADH:coenzyme Q oxidoreductase isolated from bovine heart mitochondria. Nakashima Y, Shinzawa-Itoh K, Watanabe K, Naoki K, Hano N, Yoshikawa S. J Bioenerg Biomembr. 34(1), 11-9 (2002)

2001

  • Presence of the heme-oxo intermediate in oxygenation of carbon monoxide by cytochrome c oxidase revealed by resonance Raman spectroscopy. Kim Y, Shinzawa-Itoh K, Yoshikawa S, Kitagawa T. J Am Chem Soc. 123(4), 757-8. No abstract available (2001)
  • Intermonomer interactions in dimer of bovine heart cytochrome c oxidase. Lee SJ, Yamashita E, Abe T, Fukumoto Y, Tsukihara T, Shinzawa-Itoh K, Ueda H, Yoshikawa S. Acta Crystallogr D Biol Crystallogr. 57(Pt 7), 941-7 (2001)


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タンパク質結晶成長機構兵庫県立大学 ピコバイオロジー研究所

〒678-1297
兵庫県赤穂郡上郡町
光都3-2-1
TEL 0791-58-0191
FAX 0791-58-0489