Original Paper

Hayashi, S., Mori, S., Suzuki, T., Suzuki, T., and *Yoshihisa, T. (2019) Impact of intron removal from tRNA genes on Saccharomyces cerevisiae. Nucleic Acids Res.,47:5936-5949. doi: 10.1093/nar/gkz270.

Review etc.

◯*Kanai A, Yoshihisa T. (2019) Editorial: Current Advances in the Research of RNA Regulatory Enzymes. Front. Genet., 10:973. doi: 10.3389/fgene.2019.00973.


Original Paper

Arima, K., Tamaoki, D., Mineyuki, Y., Yasuhara, H., Nakai, T., Shimmen, T., Yoshihisa, T., and *Sonobe, S. (2018) Displacement of the mitotic apparatuses by centrifugation reveals cortical actin organization during cytokinesis in cultured tobacco BY-2 cells. J. Plant Res., 131:803-815. doi: 10.1007/s10265-018-1047-4.

Review etc.

◯*Herrmann, J.M., Carvalho, P., Hayer-Hartl, M., and Yoshihisa, T. (2018) Life of proteins: from nascent chain to degradation. Nat. Struct. Mol. Biol., 25:996-999. doi: 10.1038/s41594-018-0150-5.

◯*吉久徹(2018)tRNAの成熟化と核–細胞質間ダイナミクス. Plant Morphology, 30:37-58. doi: 10.5685/plmorphol.30.37.

◯園部誠司、山岡望海2018)イカダケイソウの滑走運動機構. 生物工学, 96:257-260.





Original Paper

Yamaoka, N., Suemoto, Y., Yoshihisa, T., and *Sonobe, S. (2016) Motion analysis and ultrastructural study of a colonial diatom, Bacillaria paxillifer. Microscopy, 65:211-221. doi: 10.1093/jmicro/dfv375.

Yokota, E. (2016) Isolation of actin and actin binding proteins. Taylor, N. L. and Millar, A. H. eds., in “Isolation of Plant Organelles and Structures: Methods and Protocols,” pp. 291-300, Springer, Berlin, Germany.


Original Paper

Takano, A., Kajita, T., Mochizuki, M., Endo, T., and *Yoshihisa, T. (2015) Cytosolic Hsp70 and co-chaperones constitute a novel system for tRNA import into the nucleus. eLife, 4:e04659. doi: 10.7554/eLife.04659.

Tsuboi, T., Yamazaki, R., Nobuta, R., Ikecuhi, K., Makino, S., Ohtaki, Y., Suzuki, Y., Yoshihisa, T., Trotta, C., and *Inada, T. (2015) The tRNA splicing endonuclease complex cleaves the mitochondria-localized CBP1 mRNA. J. Biol. Chem., 290:16021-16030. doi: 10.1074/jbc.M114.634592.

Review etc.

◯*Yoshihisa, T. Nucleocytoplasmic shuttling of tRNAs and implication of the cytosolic Hsp70 system in tRNA import. Nucleus, 6:339-43. doi: 10.1080/19491034.2015.1082696.


Original Paper

Song, J., Tamura, Y., Yoshihisa, T., and *Endo, T. (2014) A novel import route for an N-anchor mitochondrial outer membrane protein aided by the TIM23 complex. EMBO Rep., 15: 670-677

Tominaga, M., Kimura, A., Yokota, E., Haraguchi, T., Shimmen, T., Yamamoto, K., Nakano, A., and *Ito, K. (2014) Cytoplasmic streaming velocity as a plant size determinant.  Dev. Cell, 27: 345-352.

Review etc.

*Yoshihisa, T. Handling tRNA introns, archaeal way and eukaryotic way. Front. Genet., 5: e00213.

*Yoshihisa, T. (2014) tRNA Subcellular Dynamics, in "Encyclopedia of Molecular Cell Biology and Molecular Medicine," ed. by Robert, A. M., pp. 513-544, Wiley-Blackwell, Mannheim, Germany.


Original Paper

Nishigami, Y., *Ichikawa, M., Kazama, T., Kobayashi, R., Shimmen, T., Yoshikawa, K., and *Sonobe, S. (2013) Reconstruction of Active Regular Motion in Amoeba Extract: Dynamic Cooperation between Sol and Gel States. PLoS ONE, 8: e70317.

Nozawa, K., Ishitani, R., Yoshihisa, T., Stao, M., Arisaka, F., Kanamaru, S., Dohmae, N., Mangroo, D., Sengrer, B., Becker, D., and *Nureki, O. : Crystal structure of Cex1p reveals the mechanism of tRNA trafficking between nucleus and cytoplasm. (2013) Nucl. Acids Res., 41: 3901-3914.

Review etc.

*Yoshihisa, T. tRNA Subcellular Dynamics, in Encyclopedia of Molecular Cell Biology and Molecular Medicine. Wiley Inc., in press.

*吉久 徹 (2013) tRNA型スプライシングの新展開. 生化学, 85: 89-91.



Mori, S., Kajita, T., Endo, T. and *Yoshihisa, T. (2011) The intron of tRNA-TrpCCA is dispensable for growth and translation of Saccharomyces cerevisiae. RNA, 17: 1760-1769 (2011)

*吉久 徹 (2011) ことば,「典型的tRNAスプライシング」, RtcB/HSPC117tRNAリガーゼ」. 生化学, 83: 1139.


Mori, T., Ogasawara, C., Inada, T., Englert, M., Beier, H., Takezawa, M., Endo, T. and *Yoshihisa, T. (2010) Dual functions of yeast tRNA ligase in the unfolded protein response: Unconventional cytoplasmic splicing of HAC1 pre-mRNA is not sufficient to release translational attenuation. Mol. Biol. Cell, 21: 3722-3734.

*Endo, T., Yamano, K., and Yoshihisa, T. (2010) Mitochondrial matrix reloaded. Cell, 142: 362-363.


Yamamoto, H., Fukui, K., Takahashi, H., Kitamura, S., Shiota, T., Terao, K., Uchida, M., Esaki, M., Nishikawa, S., Yoshihisa, T., Yamano, K., and *Endo T. (2009) Roles of TOM70 in import of presequence-containing mitochondrial proteins. J. Biol. Chem., 284: 31635-31646.

*吉久 徹 (2009) tRNAの「ダイナミクス」. 蛋白質核酸酵素増刊「mRNAプログラム」, 54:  2121-2126.


*吉久 徹 (2009) RNAの解析−whole mount in situ hybridization 3)酵母、RNA実験ノート(稲田 利文、塩見 春彦編), pp. 89-95, 羊土社.

*吉久 徹 (2009) RNAの取扱い方電気泳動RNA. 実験ノート(稲田 利文、塩見 春彦編), pp. 47-53, 羊土社.


*Yoshihisa, T., Ohshima, C., Yunoki-Esaki, K., and Endo, T. (2007) Cytoplasmic splicing of tRNA in Saccharomyces cerevisiae. Genes Cells, 12: 285-297.

*吉久 徹*遠藤 斗志也 (2007) 4.1 細胞の構造:概論. 生物物理学ハンドブック(石渡信一、桂勲、桐野豊、美宅成樹編), pp. 193-197, 朝倉書店.

*遠藤斗志也、吉久 徹、森 和俊、田口英樹 (2007) 研究の歴史:渾沌の前史から「タンパク質の一生」研究が開花するまで. タンパク質の一生:集中マスター(遠藤斗志也、森和俊、田口英樹編), pp. 12-23, 羊土社.

*遠藤斗志也、吉久 徹、森 和俊、田口英樹 (2007) ブレイクスルーとなった実験法を中心に:そのとき「タンパク質の一生」研究の歴史が動いた. タンパク質の一生:集中マスター(遠藤斗志也,森和俊,田口英樹編), pp. 25-35, 羊土社.


*Yoshihisa, T. (2006) tRNA, new aspects in intracellular dynamics. Cell. Mol. Life Sci., 63: 1813-1818.

*吉久 徹* (2006) 細胞質間を行き来するtRNA. 蛋白質核酸酵素, 51: 48-53.

*吉久 徹,高野 晃,遠藤 斗志也 (2006) 核内tRNAの生理的意義. 蛋白質核酸酵素増刊「細胞核の世界」, 51: 2232-2234.

*吉久 徹 (2006) tRNAの成熟化と細胞内ダイナミクス−tRNAの一生における新展開. 蛋白質核酸酵素増刊「RNAと生命」. 51: 2568-2573.


Takano, A.. Endo, T., and *Yoshihihsa, T. (2005) tRNA actively shuttles between the nucleus and cytosol in yeast. Science, 309: 140-142.


◯遠藤 斗志也*吉久 徹 (2004) 総論:タンパク質の移動(真核生物)細胞内の交通管制システムがはたらく仕組み. 蛋白質核酸酵素増刊「細胞における蛋白質の一生」, 49: 877-888.


*Yoshihisa, T., Yunoki-Esaki, K., Ohshima, C., Tanaka, N., and Endo, T. (2003) Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria. Mol. Biol. Cell, 14: 3266-3279.

*遠藤 斗志也、江崎 雅俊、山本 林、吉久 徹 (2003) ミトコンドリアをめぐる蛋白質フラックス. 実験医学増刊「細胞内輸送」, 21: 1889-1895.


吉久 徹*遠藤 斗志也 (2002) 葉緑体包膜およびチラコイド膜を介する物質輸送とその制御. 朝倉植物生理学講座3-光合成, pp. 109-124, 朝倉書店.


Kurihara, T., Hamamoto, S., Gimeno, R. E. , Kaiser, C. A. , *Schekman, R., and Yoshihisa, T.: Sec24p and Iss1p function interchangeably in transport vesicle formation from the endoplasmic reticulum in Saccharomyces cerevisiae. Mol. Biol. Cell, 11: 983-998.

吉久 徹、遠藤 斗志也* (2000) 葉緑体における蛋白質の輸送. 蛋白質核酸酵素 45pp. 139-146、共立出版 (2000)

T. Asai, Y. Shinoda, T. Nohara, T. Yoshihisa*, and T. Endo: Sec-dependent pathway and ∆pH-dependent pathway do not share a common translocation pore in thylakoidal protein transport. J. Biol. Chem. 274, 20075-20078 (1999)

吉久 徹*:オルガネラの特異的単離法,酵母ラボマニュアル(山本 正幸,大矢禎一編) pp. 149-151 シュプリンガー・フェアラーク東京(1999

T. Homma, T. Yoshihisa, and K. Ito*: Subunit interactions in the Escherichia coli protein translocase: SecE and SecG associate independently with SecY. FEBS lett. 408, 11-15 (1997)

M. Sone, S. Kishigami, T. Yoshihisa, and K. Ito*: Roles of disulfide bonds in bacterial alkaline phosphatase. J. Biol. Chem. 272, 6174-6178 (1997)

G. Matsumoto, T. Yoshihisa, and K. Ito*: SecY and SecA interact to allow SecA insertion and protein translocation across the E. coli plasma membrane. EMBO J. 16, 6384-6393 (1997)

T. Taura, T. Yoshihisa, and K. Ito*: Protein translocation functions of Escherichia coli SecY: in vitro characterization of cold-sensitive secY mutants. Biochimie 79, 51-72 (1997)

T. Yoshihisa, and K. Ito*: Pro-OmpA derivatives with a His6 tag in their N-terminal "translocation initiation domains" are arrested by Ni2+ at an early post-targeting stage of translocation. J. Biol. Chem. 271, 9429-9436 (1996)

T. Shimoike, T. Taura, A. Kihara, T. Yoshihisa, Y. Akiyama, K. Cannon, and K. Ito*: Product of a new gene, syd, functionally interacts with SecY when overproduced in Escherichia coli. J. Biol. Chem. 270, 5519-5526 (1995)

T. Homma, T. Yoshihisa, A. Kihara, Y. Akiyama, and K. Ito*: Intracellular stability of alpha-fragments of beta-galactosidase: Effect of amino-terminally fused polypeptides. Biochem. Biophys. Res. Commun. 215, 453-458 (1995)

T. Yeung, T. Yoshihisa, and R. Schekman*: Purification of Sec23p-Sec24p Complex. Methods in Enzymol. 257, 145-151 (1995)

T. Yoshihisa, C. Barlowe, and R. Schekman*: Sec23, in Guidebook to the Small GTPases (M. Zerial, and L. A. Huber ed.) pp452-453, Oxford University Press, Oxford (1995)

T. Baba, T. Taura, T. Shimoike, Y. Akiyama, T. Yoshihisa, and K. Ito*: A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex. Proc. Natl. Acad. Sci. USA 91, 4539-4543 (1994)

T. Yoshihisa*: SEC24, in Guidebook to the Secretory Pathway (J. Rothblatt, P. Novick, and T. Stevens ed.) pp120-121, Oxford University Press, Oxford (1994)

T. Yoshihisa, C. Barlowe, and R. Schekman*: Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum. Science 259, 1466-1468 (1993)

L. Hicke, T. Yoshihisa, and R. Schekman*: Sec23p and a novel 105-kDa protein function as a multimeric complex to promote vesicle budding and protein transport from the endoplasmic reticulum Mol. Biol. Cell. 3, 667-676 (1992)

L. Hicke, T. Yoshihisa, and R. Schekman*: Purification of yeast Sec23 protein by complementation of mutant cell lysates deficient in endoplasmic reticulum-to-Golgi transport. Methods in Enzymol. 219, 338-352 (1992)

吉久 徹、安楽 泰宏*: 酵母液胞へのタンパク質輸送、新生化学実験講座生態膜と膜輸送(下)6巻(日本生化学会編)pp. 689-696、東京化学同人(1992

T. Yoshihisa, and Y. Anraku*: A novel pathway of import of lpha-mannosidase, a marker enzyme of vacuolar membrane, in Saccharomyces cerevisiae. J. Biol. Chem. 265, 22418-22425 (1990)

N. Umemoto, T. Yoshihisa, R. Hirata, and Y. Anraku*: Roles of the VMA3 gene product, subunit c of the vacuolar membrane proton-ATPase on vacuolar acidification and protein transport: a study with VMA3-disrupted mutants of Saccharomyces cerevisiae. J. Biol. Chem. 265, 18447-18453 (1990)

T. Yoshihisa, and Y. Anraku*: Nucleotide sequence of AMS1, the structure gene of vacuolar Alpha-mannosidase of Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 163, 908-915 (1989)

T. Yoshihisa, Y. Ohsumi, and Y. Anraku*: Solubilization and purification of alpha-mannosidase, a marker enzyme of vacuolar membranes in Saccharomyces cerevisiae. J. Biol. Chem. 263, 5158-5163 (1988)

S. Amino, T. Yoshihisa, and A. Komamine*: Changes in glucan synthase activities during the cell cycle in a synchronous culture of Catharanthus roseus. Physiol. Plant. 65, 67-71 (1985)