研究成果

Sayer, J.A., Otto, E.A., O'toole, J.F., Nurnberg, G., Kennedy, M.A., Becker, C., Hennies, H.C., Helou, J., Attanasio, M., Fausett, B.V., Utsch, B., Khanna, H., Liu, Y., Drummond, I., Kawakami, I., Kusakabe, T., Tsuda, M., Ma, L., Lee, H., Larson, R.G., Allen, S.J., Wilkinson, C.J., Nigg, E.A., Shou, C., Lillo, C., Williams, D.S., Hoppe, B., Kemper, M.J., Neuhaus, T., Parisi, M.A., Glass, I.A., Petry, M., Kispert, A., Gloy, J., Ganner, A., Walz, G., Zhu, X., Goldman, D., Nurnberg, P., Swaroop, A., Leroux, M.R., and Hildebrandt, F.
The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4
Nat. Genet. 38 (6), 674-681.

Takimoto, N., Kusakabe, T., Horie, T., Miyamoto, Y., and Tsuda, M.
Origin of the vertebrate visual cycle: III. Distinct distribution of RPE65 and beta-carotene 15,15'-monooxygenase homologues in Ciona intestinalis
Photochem. Photobiol., in press (published online 17 March 2006)

Sierro, N., Kusakabe, T., Park, K.-J., Yamashita, R., Kinoshita, K., and Nakai, K.
DBTGR: a database of tunicate promoters and their regulatory elements
Nucleic Acids Res. 34, D552-D555 (2006)

Barran, P.E., Roeske, R.W., Pawson, A.J., Sellar, R., Bowers, M.T., Morgan, K., Lu, Z.-L., Tsuda, M., Kusakabe, T., and Millar, R.P.
Evolution of constrained GnRH ligand conformation and receptor sensitivity
J. Biol. Chem. 280 (46), 38569-38575 (2005)

Otto, E.A., Loeys, B., Khanna, H., Hellemans, J., Sudbrak, R., Fan, S., Muerb, U., O'Toole, J.F., Helou, J., Attanasio, M., Utsch, B., Sayer, J.A., Lillo, C., Jimeno, D., Coucke, P., De Paepe, A., Reinhardt, R., Klages, S., Tsuda, M., Kawakami, I., Kusakabe, T., Omran, H., Imm, A., Tippens, M., Raymond, P.A., Hill, J., Beales, P., He, S., Kispert, A., Margolis, B., Williams, D.S., Swaroop, A., and Hildebrandt, F.
A novel ciliary IQ domain protein, NPHP5, is mutated in Senior-Loken syndrome (nephronophthisis with retinitis pigmentosa), and interacts with RPGR and calmodulin
Nat. Genet. 37 (3), 282-288 (2005)

Jiang, D., Tresser, J.W., Horie, T., Tsuda, M., and Smith, W.C.
Pigmentation in the sensory organs of the ascidian larva is essential for normal behavior
J. Exp. Biol. 208 (Pt 3), 433-438 (2005)

Kusakabe, T., Yoshida, R., Ikeda, Y., and Tsuda, M.
Computational discovery of DNA motifs associated with cell type-specific transcription in Ciona
Dev. Biol. 276 (2), 563-580 (2004)

Sakurai, D., Goda, M., Koumura, Y., Horie, T., Iwamoto, H., Ohtsuki, H., and Tsuda, M.
The role of pigment cells in the brain of ascidian larva
J. Comp. Neurol. 475 (1), 70-82 (2004)

Yoshida, R., Sakurai, D., Horie, T., Kawakami, I., Tsuda, M., and Kusakabe, T.
Identification of neuron-specific promoters in Ciona intestinalis
Genesis 39 (2), 130-140 (2004)

Ando, H., Shi, Q., Kusakabe, T., Ohya, T., Suzuki, N., and Urano, A.
Localization of mRNAs encoding alpha and beta subunits of soluble guanylyl cyclase in the brain of rainbow trout: comparison with the distribution of neuronal nitric oxide synthase
Brain Res. 1013 (1), 13-29 (2004)

Ashida, A., Matsumoto, K., Ebrey, T.G., and Tsuda, M.
A purified agonist-activated G-protein coupled receptor: truncated octopus acid metarhodopsin
Zool. Sci. 21 (3), 245-250 (2004)

Kusakabe, T., Mishima, S., Shimada, I., Kitajima, Y., and Tsuda, M.
Structure, expression, and cluster organization of genes encoding gonadotropin-releasing hormone receptors found in the neural complex of the ascidian Ciona intestinalis
Gene 322, 77-84 (2003)

Tsuda, M., Kusakabe, T., Iwamoto, H., Horie, T., Nakashima, Y., Nakagawa, M., and Okunou, K.
Origin of the vertebrate visual cycle. II. Visual cycle proteins are localized in whole brain including photoreceptor cells of a primitive chordate
Vision Res. 43 (28), 3045-3053 (2003)

Inada, K., Horie, T., Kusakabe, T., and Tsuda, M.
Targeted knockdown of an opsin gene inhibits the swimming behaviour photoresponse of ascidian larvae
Neurosci. Lett. 347 (3), 167-170 (2003)

Nakashima, Y., Kusakabe, T., Kusakabe, R., Terakita, A., Shichida, Y., and Tsuda, M.
Origin of the vertebrate visual cycle: genes encoding retinal photoisomerase and two putative visual cycle proteins are expressed in whole brain of a primitive chordate
J. Comp. Neurol. 460 (2), 180-190 (2003)

Tsuda, M., Sakurai, D., and Goda, M.
Direct evidence for the role of pigment cells in the brain of ascidian larvae by laser ablation
J. Exp. Biol.,206(8), 1409-1417 (2003)

Tsuda, M., Kawakami, I., and Shiraishi, S.
Sensitization and habituation of the swimming behavior in ascidian larvae to light
Zool. Sci. 20, 13-22 (2003)

Iwasa, T., Mishima, S., Watari, A., Ohkuma, M., Azuma, T., Kanehara, K., and Tsuda. M.
A novel G protein alpha-subunit in embryo of the ascidian, Halocynthia roretzi
Zool. Sci. 20, 141-152 (2003)

Yoshida, R., Kusakabe, T., Kamatani, M., Daitoh, M., and Tsuda, M.
Central nervous system-specific expression of G protein alpha-subunit isoforms in the ascidian Ciona intestinalis
Zool. Sci. 19 (10), 1079-1088 (2002)

Nakagawa, M., Orii, H., Yoshida, N., Jojima, E., Horie, T., Yoshida, R., Haga , T., and Tsuda, M.
Ascidian arrestin (Ci-arr), the origin of the visual and nonvisual arrestins of vertebrate
Eur. J. Biochem. 269 (21), 5112-5118 (2002)

Kusakabe, T., Nakashima, Y., Kusakabe, R., Takeo, H., Kawakami, I., Yoshida, R., Inada, K., Nakagawa, M., and Tsuda, M.
Phototransduction and visual cycle in the ascidian tadpole larva
J. Photosci. 9 (2), 37-40 (2002)

Horie,T., Nakagawa, M., Orii, H., and Tsuda, M.
Whole structure of the photoreceptors in the ascidian larva visualized by an antibody against arrestin (Ci-Arr)
J. Photosci. 9 (2), 272-274 (2002)

Satou, Y., Takatori, N., Fujiwara, S., Nishikata, T., Saiga, H., Kusakabe, T., Shin-i, T., Kohara, Y., and Satoh, N.
Ciona intestinalis cDNA projects: EST analyses and gene expression profiles during embryogenesis
Gene 287 (1-2), 83-96 (2002)

Nishioku, Y., Nakagawa, M., Tsuda, M., and Terazima, M.
Energetics and volume changes of the intermediates in the photolysis of octopus rhodopsin at a physiological temperature
Biophys. J. 83 (2), 1136-1146 (2002)

Kawakami,I., Siraishi,I., and Tsuda, M.
Photoresponse and learning behavior of ascidian larvae, a primitive chordate, to repeated stimuli of step-up and step-down of light
J. Biol. Physic. 28, 1-11(2002)

Okano, K., Oishi,T., Miyashita, Y., Moriya, T., Tsuda, M., Irie,T., Ueki, N., and Seki, T.
Identification of 3,4-didehydroretinal Isomers in photosensitive melanophores of the Xenopus tadpole tail fin
Zool. Sci. 19, 191-195 (2002)

Kusakabe, T., Yoshida, R., Kawakami, I., Kusakabe, R., Mochizuki, Y., Yamada, L., Shin-i, T., Kohara, K., Satoh, N., Tsuda, M., and Satou, Y.
Gene expression profiles in tadpole larvae of Ciona intestinalis
Dev. Biol. 242 (2),188-203 (2002)

Kusakabe, T., Kusakabe, R., Kawakami, K., Satou, Y., Satoh, N., and Tsuda, M.
Ci-opsin1, a vertebrate-type opsin gene, expressed in the larval ocellus of the ascidian Ciona intestinalis
FEBS Lett. 506 (1), 69-72 (2001)

Kuriyama, H., Nakagawa, M., and Tsuda, M.
Intracellular Ca2+ changes induced by in vitro ischemia in rat retinal slices
Exp. Eye Res. 73 (3),365-374 (2001)

Nakagawa, M. Miyamoto,T., Kusakabe, R., Takasaki, S., Takao,T., Shichida ,Y., and Tsuda, M.
O-glycosylation of G-protein coupled receptor, octopus rhodopsin
FEBS Lett. 496, 19-24 (2001)

Nishioku,Y., Nakagawa, M., Tsuda, M., and Terazima,M.
A spectrally silent transformation in the photolysis of octopus rhodopsin: a protein conformational change without any accompanying change of the chromophore's absorption
Biophys. J. 80 (6), 2922-2927 (2001)

Kuriyama, H., Waki, M., Nakagawa, M., and Tsuda, M.
Involvement of oxygen free radicals and intracellular calcium dynamics in experimental retinal ischemia
Ophthalmic Res. 33 (4),196-202 (2001)

Kusakabe, T. and Suzuki, N.
A cis-regulatory element essential for photoreceptor-specific expression of a medaka retinal guanylyl cyclase gene
Dev. Genes Evol. 211 (3), 145-149 (2001)

Tsuda, M., Ohkuma, M., Nakagawa, M., and Katagiri, Y.
Light regulated GnRH neurons in biological clock for reproduction in the ascidin, Halocynthia roretzi
The Biology of Ascidians (Springer) pp 131-136 (2001)

Iwasa, T., Kanehara, K., Watari, A., Ohkuma, M., Nakagawa, M., and Tsuda, M.
Hererotrimc G protein alpha and beta subunit genes of the ascidian, Halocynthia roretzi
The Biology of Ascidians (Springer) pp 147-152 (2001)

Tsuda,M., Kawakami,I., Miyamoto,T., Nakagawa,M., Shiraishi, S., and Gouda,M.
Photoresponse and habituation of swimming behavior of ascidian larvae, Ciona intestinalis
The Biology of Ascidians (Springer) pp 153-157 (2001)

Kusakabe, T.
Evolution of anural development in ascidians: role of muscle-specific differentiation genes
The Biology of Ascidians (Springer) pp 225-229 (2001)

Kusakabe, T. and Suzuki, N.
Photoreceptors and olfactory cells express the same retinal guanylyl cyclase isoform in medaka: visualization by promoter
transgenics. FEBS Lett. 483 (2-3), 143-148 (2000)

Iwasa, T., Yanai, T., Nakagawa, M., Kikkawa, S., Obata, S., Usukura, J., and Tsuda, M.
G Protein a Subunit Genes in Octopus Photoreceptor Cells
Zool. Sci. 17, 711-716 (2000)

Ohkuma, M., Katagiri, Y., Nakagawa, M., and Tsuda, M.
Possible involvement of light regulated gonadotropin-releasing hormone neurons in biological clock for reproduction in the cerebral ganglion of the ascidian, Halocynthia roretzi
Neurosci. Lett. 293 (1), 5-8 (2000)

Ohkuma, M. and Tsuda, M.
Visualization of retinal proteins in the cerebral ganglion of ascidian, Halocynthia roretzi
Zool. Sci. 17 (2), 161-170 (2000)

Nakagawa, M., Miyamoto, T., Ohkuma, M., and Tsuda, M.
Action Spectrum for the Photoresponse of Ciona intestinails (Ascidiece, Urchodata) Larva Implicates Retinal Protein
Photochem. Photbiol. 70 (3), 359-362 (1999)

Nakagawa,M., Kikkawa,S., Iwasa,T., Tsuda, M., and Ebrey, T.G.
How vertebrate and invertebrate visual pigments differ in their mechanism of photoactivation
Proc. Natl. Acad. Sci. USA 96, 6189-6192 (1999)

Nakagawa,M., Kikkawa,S., Tominaga, K., Tsugi, N., and Tsuda, M.
A novel photointermediate of octopus rhodopsin activates its G-protein
FEBS Lett. 436, 259-262 (1998)

Ohguro, H., Toshida, N., Shindou, H., and M. Tsuda
Identification of a single Phosphorylation site within octopus rhodopsin
Photochem. Photbiol. 60 (6), 824-828 (1998)

Iwasa, T., L. Colmenares, K. Hirata, Y. Arime, M. Nakagawa, S. Kikkawa, H. Takashima, A. Nosaka, A. Naito, H. Saito, R. Liu, and M. Tsuda
NMR and UV-VIS absorption spectroscopic studies on fluorinated octopus rhodopsin and its photoproducts
J. Phys. Chem. B 102, 5602-5610 (1998).

T. Kobayashi, M. Kim, M. Taiji, T. Iwasa, M. Nakagawa, and M. Tsuda
Femtosecond spectroscopy of halorhodopsin and rhodopsin in a broad spectral range of 400-1000 nm
J. Phys. Chem. B 102, 272-280 (1998)

Kikkawa, S., Yoshida, N., Nakagawa, M., Iwasa, and Tsuda, M.　
A Novel Rhodopsin Kinase in Octopus Photoreceptor Possesses a Pleckstrin Homology Domain and is activated by G protein bg Subunits
J. Biol. Chem. 273 (13), 7441-7447 (1998)

Zhang,Y., Iwasa, T., Tsuda, M., Kobata, A., and Takasaki, S.
A novel monoantennary complex-type sugar chain found in octopus rhodopsin: occurrence of the Gal_->4Fuc group linked to the proximal N-acetylglucosamine residue of the trimannosyl core
Glycobiol. 7 (8) 1153-1158 (1997)

Haung, L., Deng, H., Koutalos, Y., Ebrey, T., Groesbeek, M., Lugtenburg, J., Tsuda, M., and Callender, R.H.
A Resonance Raman Study of the C=C Strech Modes in Bovine and Octopus Visual Pigments with Isotopically Labeled Retinal Chromophores
Photochem. Photobiol. 66 (6), 747-754 (1997)

Nakagawa, M., Iwasa, T., Kikkawa, S.,Takao, T., Shimonishi, Y., and Tsuda, M.
Identification of Two Palmitoyl Groups in Octopus Rhodopsin
Photochem. Photobiol. 65 (1),187-191 (1997)

Nishimura, S., Kandori, H., Nakagawa,M., Tsuda, M., and Maeda, A.
Structural Dynamics of Waters and the Peptide Backbone around the Schiff Base Associated with the Light -activated Process of Octopus Rhodopsin
Biochemistry 36 (4), 864-870 (1997)

Nakagawa, M., Kikkawa, K., Iwasa , T., and Tsuda. M.
Light-induced Protein Conformational Changes in the Photolysis of Octopus Rhodopsin
Biophys. J. 72 (5), 2320-2328 (1997)

Kikkawa, S., Tominaga, K., Nakagawa, N., Iwasa, I., and Tsuda, M.
Simple Purification and Functional Reconstitution of Octopus Photoreceptor Gq, Which Couples Rhodopsin to Phospholipase C
Biochemistry 35 (49),15857-15864 (1996)

Hashimoto, S., Takeuchi, H., Nakagawa, M., and Tsuda, M.
Ultraviolet Resonance Raman Evidence for the Absence of Tyrosine in Octopus Rhodopsin and the Participation of Trp residues in the Transition to Acid Metarhodopsin
FEBS Lett. 398 (2-3), 239-242 (1996)

Huang, L., Deng, H., Weng, G., Koutalos, Y., Ebrey, T., Groesbeek, M., Lugtenburg, J., Tsuda, M., and Callender, R.H.
A Resonance Raman Study of the C=N Configuration of Octopus Rhodopsin, Bathorhodopsin, and Isorhodopsin
Biochemistry 35, 8505-8510 (1996)

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