発表論文（2006 ~2010 ）

発表論文（2000 ~ 2005）

Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.

Ishii T, Shiomi Y, Takami T, Murakami Y, Ohnishi N, Nishitani H.

J Biol Chem. 285(53):41993-2000. 2010

[Function of multiple clamp and loader systems in eukaryotic chromosome replication].

Shiomi Y, Tsurimoto T.

Seikagaku. 81(5):386-9. 2009

START-GAP2/DLC2 is localized in focal adhesions via its N-terminal region.

Kawai K, Seike J, Iino T, Kiyota M, Iwamae Y, Nishitani H, Yagisawa H.

Biochem Biophys Res Commun. 380(4):736-741, 2009.

[Control of DNA replication: how to limit replication in a cell cycle].

Nishitani H.

Seikagaku. 80(7):661-6. Review. 2008

CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation.

Nishitani H, Shiomi Y, Iida H, Michishita M, Takami T, Tsurimoto T.

J Biol Chem. 283(43):29045-52. 2008

Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity.

Ohtsubo M, Yasunaga S, Ohno Y, Tsumura M, Okada S, Ishikawa N, Shirao K, Kikuchi A, Nishitani H, Kobayashi M, Takihara Y.

Proc Natl Acad Sci U S A. 105(30):10396-401. 2008

Schizosaccharomyces pombe Snf2SR, a novel SNF2 family protein, interacts with Ran GTPase and modulates both RanGEF and RanGAP activities.

Ohba T, Nishijima H, Nishitani H, Nishimoto T.

Genes Cells. 13(6):571-82. 2008

Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction.

Oki M, Ma L, Wang Y, Hatanaka A, Miyazato C, Tatebayashi K, Nishitani H, Uchida H, Nishimoto T.

Gene. 400(1-2):114-121. 2007

A second proliferating cell nuclear antigen loader complex, Ctf18-replication factor C, stimulates DNA polymerase eta activity.

Shiomi Y, Masutani C, Hanaoka F, Kimura H, Tsurimoto T.

J Biol Chem. 282(29):20906-20914. 2007

Cdt1 associates dynamically with chromatin throughout G1 and recruits Geminin onto chromatin.

Xouri G, Squire A, Dimaki M, Geverts B, Verveer PJ, Taraviras S, Nishitani H, Houtsmuller AB, Bastiaens PI, Lygerou Z.

EMBO J. 26(5):1303-14. 2007

Licensing regulators Geminin and Cdt1 identify progenitor cells of the mouse CNS in a specific phase of the cell cycle.

Spella M, Britz O, Kotantaki P, Lygerou Z, Nishitani H, Ramsay RG, Flordellis C, Guillemot F, Mantamadiotis T, Taraviras S.

Neuroscience. 147(2):373-387, 2007

Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit Brahma.

Roukos V, Iliou MS, Nishitani H, Gentzel M, Wilm M, Taraviras S, Lygerou Z.

J Biol Chem. 282(13):9346-57. 2007

Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway.

Hayashi N, Murakami S, Tsurusaki S, Nagaura Z, Oki M, Nishitani H, Kobayashi M, Shimizu H,  Yamamoto K, Nishimoto T.

Biochem Biophys Res Commun. 353(2): 330-336. 2007

Identification of a heat-shock protein Hsp40, DjB1, as an acrosome- and a tail-associated component in rodent spermatozoa.

Doiguchi M, Kaneko T, Urasoko A, Nishitani H, Iida H.

Mol Reprod Dev. 74(2): 223-232, 2007

Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe.

Nishijima H, Nakayama J, Yoshioka T, Kusano A, Nishitani H, Shibahara K, Nishimoto T

Mol. Biol. Cell 17: 2524-2536, 2006

Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis.

Nishitani H, Sugimoto N, Roukos V, Nakanishi Y, Saijo M, Obuse C, Tsurimoto T, Nakayama KI, Nakayama K, Fujita M, Lygerou Z, Nishimoto T.

EMBO J. 25(5):1126-36. 2006

Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombe.

Hirose E, Mukai M, Shimada A, Nishitani H, Shibata Y, Nishimoto T

Genes Cells 11(1): 29-46, 2006

*****

Mitotic UV irradiation induces a DNA replication-licensing defect that potentiates G1 arrest response.

Morino M, Nukina K, Sakaguchi H, Maeda T, Takahara M, Shiomi Y, Nishitani H.

PLoS One. 2015 Mar 23;10(3):e0120553.

RanBP9 Modulates AICD Localization and Transcriptional Activity via Direct Interaction with Tip60.

Domingues SC, Konietzko U, Henriques AG, Rebelo S, Fardilha M, Nishitani H, Nitsch RM, da Cruz E Silva EF, da Cruz E Silva OA.

J Alzheimers Dis. 42(4):1415-33, 2014

Imaging analysis to determine chromatin binding of licensing factors MCM2-7 in mammalian cells.

Mayayuki Morino, Miyuki Tanaka, Yasuhi Shiomi, Hideo Nishitani

Methods Mol Biol. 1170:529-37, 2014

PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells.

Akiyo Hayashi, Naohiro Suenaga, Yasushi Shiomi and Hideo Nishitani.

Methods Mol Biol. 1170:367-82, 2014

Imaging analysis of cell cycle-dependent degradation of Cdt1 in mammalian cells.

Yasushi Shiomi, Naohiro Suenaga, Miyuki Tanaka, Akiyo Hayashi, and Hideo Nishitani

Methods Mol Biol. 1170:357-65, 2014

Chromatin fractionation analysis of licensing factors in mammalian cells.

Hideo Nishitani, Masayuki Morino, Yusuke Murakami, Takeshi Maeda, Yasushi Shiomi

Methods Mol Biol. 1170:517-27, 2014

Alternative replication factor C protein, Elg1, maintains chromosome stability by regulating PCNA levels on chromatin

Shiomi Y, Nishitani H

Genes Cells. 18(11):946-59, 2013

Cell Cycle-dependent Subcellular Translocation of the Human DNA Licensing Inhibitor Geminn

Dimaki M, Xouri G, Symeonidou IE, Sirinian C, Nishitani H, Taraviras S, Lygerou Z.

J Biol Chem. 288(33):23953-63, 2013

Inhibition of DNA Damage-Induced Apoptosis through Cdc7-Mediated Stabilization of Tob.

Suzuki, T, Tsuzuku, J, Hayashi,A, Shiomi, Y, Iwanari, H, Mochizuki,Y, Hamakubo,T., Kodama,T, Nishitani H, Masai, H, and Yamamoto, T

J Biol Chem. 287(48):40256-65. 2012

Checkpoint Kinase ATR Phosphorylates Cdt2, a Substrate Receptor of CRL4 Ubiquitin Ligase, and Promotes the Degradation of Cdt1 following UV-irradiation.

Sakaguchi,H, Takami,T, Yasutani,Y, Maeda,T, Morino,M, Ishii,T, Shiomi,Y, Nishitani, H .

PLoS ONE 7(9): e46480, 2012

Two different RFC proteins, Ctf18 and RFC1 separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV-irradiation.

Shiomi Y, Hayashi A, Ishii T, Shinmyozu K, Nakayama JI, Sugasawa K, Nishitani H.

Mol Cell Biol. 32(12):2279-2288. 2012

Cdt1 is differentially targeted for degradation by anticancer chemotherapeutic drugs

Stathopoulou A, Roukos V, Petropoulou C, Kotsantis P, Karantzelis N, Nishitani H, Lygerou Z, Taraviras S.

PLoS One. 7(3):e34621. 2012

Dynamic recruitment of licensing factor Cdt1 to sites of DNA damage.

Roukos V, Kinkhabwala A, Colombelli J, Kotsantis P, Taraviras S, Nishitani H, Stelzer E, Bastiaens P, Lygerou Z.

J Cell Sci. 124(Pt 3):422-34. 2011

Positively charged residues located downstream of PIP box, together with TD amino acids within PIP box, are important for CRL4(Cdt2) -mediated proteolysis.

Michishita M, Morimoto A, Ishii T, Komori H, Shiomi Y, Higuchi Y, Nishitani H.

Genes Cells. 16(1):12-22. 2011

発表論文（2010 ~2015 ）

発表論文（2016 ~ 2020）

*****

CRL4Cdt2: Coupling Genome Stability to Ubiquitination.

Panagopoulos A, Taraviras S, Nishitani H, Lygerou Z. Trends Cell Biol. 2020 Apr;30(4):290-302. doi: 10.1016/j.tcb.2020.01.005. Epub 2020 Feb 7.

A DNA-binding domain in the C-terminal region of Cdt2 enhances the DNA synthesis-coupled CRL4Cdt2 ubiquitin ligase activity for Cdt1.

Mazian MA, Suenaga N, Ishii T, Hayashi A, Shiomi Y, Nishitani H. J Biochem. 2019 Jun 1;165(6):505-516. doi: 10.1093/jb/mvz001.

Direct binding of Cdt2 to PCNA is important for targeting the CRL4Cdt2 E3 ligase activity to Cdt1

Hayashi A, Giakoumakis NN, Heidebrecht T, Ishii T, Panagopoulos A, Caillat C, Takahara M, Hibbert RG, Suenaga N, Stadnik-Spiewak M, Takahashi T, Shiomi Y, Taraviras S, von Castelmur E, Lygerou Z, Perrakis A, Nishitani H. Life Sci Alliance. 2018 Dec 31;1(6):e201800238. doi: 10.26508/lsa.201800238.

Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system Wright GSA, Saeki A, Hikima T, Nishizono Y, Hisano T, Kamaya M, Nukina K, Nishitani H, Nakamura H, Yamamoto M, Antonyuk SV, Hasnain SS, Shiro Y, Sawai H. Sci Signal. 2018 Apr 10;11(525). pii: eaaq0825. doi: 10.1126/scisignal.aaq0825.

Mutations at multiple CDK-phosphorylation consensus sites on Cdt2 increase the affinity of CRL4Cdt2 for PCNA and its ubiquitination activity in S phase

Nukina K, Hayashi A, Shiomi Y, Sugasawa K, Ohtsubo M, Nishitani H. Genes Cells

23(3):200-213. doi: 10.1111/gtc.12563. Epub 2018 Feb 9. 2018

Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair

Niida H, Matsunuma R, Horiguchi R, Uchida C, Nakazawa Y, Motegi A, Nishimoto K, Sakai S, Ohhata T, Kitagawa K, Moriwaki S, Nishitani H, Ui A, Ogi T, Kitagawa M.

Nat Commun. 2017 Jul 18;8:16102. doi: 10.1038/ncomms16102.

Thymine DNA glycosylase modulates DNA damage response and gene expression by base excision repair-dependent and independent mechanisms.

Nakamura T, Murakami K, Tada H, Uehara Y, Nogami J, Maehara K, Ohkawa Y, Saitoh H, Nishitani H, Ono T, Nishi R, Yokoi M, Sakai W, Sugasawa K.

Genes Cells. 2017 Apr;22(4):392-405. doi: 10.1111/gtc.12481. Epub 2017 Mar 20.PMID: 28318075

Mismatch repair proteins recruited to ultraviolet light-damaged sites lead to degradation of licensing factor Cdt1 in the G1 phase.

Tanaka M, Takahara M, Nukina K, Hayashi A, Sakai W, Sugasawa K, Shiomi Y, Nishitani H.

Cell Cycle. 2017 Apr 3;16(7):673-684. doi: 10.1080/15384101.2017.1295179. Epub 2017 Feb 22.

PMID: 28278049

Control of Genome Integrity by RFC Complexes; Conductors of PCNA Loading onto and Unloading from Chromatin during DNA Replication.

Shiomi Y, Nishitani H.

Genes (Basel). 2017 Jan 26;8(2). pii: E52. doi: 10.3390/genes8020052. Review. PMID: 28134787

発表論文 1999以前

Self-organization of microtuble asters induced in Xenopus egg extracts by GTP-bound Ran.

Ohba T, Nakamura M, Nishitani H, Nishimoto T

Science 284: 1356-1358, 1999

When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic nucleation similar to g tubulin

Nakamura M, Masuda v, Horii J, Kuma K, Yokoyama N, Ohba T, Nishitani H, Miyata T, Tanaka M, Nishimoto T

10. J.Cell Biol. 143: 1041-1052, 1998
    

Cdc18 transcription and proteolysis couple S phase to passage through mitosis.

Baum B, Nishitani H, Yanow S, Nurse P

EMBO J. 17: 5689-5698, 1998

Cdc18p can block mitosis by two independent mechanisms.

Greenwood E, Nishitani H, Nurse P

J Cell Sci. 111: 3101-3108, 1998

A dual- specificity phosphatase Cdc25B is an unstable protein and triggers p34(cdc2)/cyclin B activation in hamster BHK21 cells arrested with hydroxyurea.

Nishijima H, Nishitani H, Seki T, Nishimoto T

10. J.Cell Biol. 138: 1105-1116, 1997
    

The cdc18 protein initiates DNA replication in fission yeast.

Nishitani H, Nurse P

Progress Cell Cycle Research. 3: 135-142, 1997

A hamster temperature-sensitive G1 mutant, tsBN250 has a single point mutation in histidyl-tRNA synthetase that inhibits an accumulation of cyclinD1.

Motomura S, Fukushima K, Nishitani H, Nawata H, Nishimoto T

Genes Cells 1:1101-1112, 1996

p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast.

Nishitani H, Nurse P

Cell 83: 397-405, 1995

Detachment of cultured cells from the substratum induced by the neutrophi-derived oxidant NH2Cl: Synergetic role of phosphotyrosine and intracellular Ca2+ concentration.

Nakamura TY, Yamamoto I, Nishitani H, Matozaki T, Suzuki T, Wakabayashi S, Shigematu M, Goshima K

10. J.Cell Biol. 131: 509-524, 1995
    

A new type of E. coli recombinational hotspot which requires for activity both DNA replication termination events and the Chi sequence.

Horiuchi T, Nishitani H, Kobayashi T

Adv Biophys. 31: 133-147, 1995

The DNA replication fork blocked at the Ter site may be an entrance for the RecBCD enzyme into Duplex DNA.

Horiuchi T, Hujimura Y, Nishitani H, Kobayashi T, Hidaka M

J. Bacteriol. 176: 4656-4663, 1994

Specific chromosome sites enhancing homologous recombination in Escherichia coli mutants defective in RNaseH.

Nishitani H, Hidaka M, Horiuchi T

Mol. Gen. Genet. 240: 307-314, 1993

Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm.

Seki T, Yamashita K, Nishitani H, Takagi T, Russell P, Nishimoto T

Mol. Biol. Cell 3: 1373-1388, 1992

RCC1, a regulatorof mitosis is essential for DNA replication.

Dasso M, Nishitani H, Kombluth S, Nishimoto T, Newport J

Mol. Cell. Biol. 12: 3337-3345, 1992

The tsBN75 and tsBN423, temperature sensitive X-linked mutants of BHK21 cell can be complemented by the ubiquitin-activating enzyme, E1 cDNA.

Nishitani H, Goto H, Kaneda S, Yamano F, Seno T, Handley P, Schwartz A, Nishimoto T

BBRC 184: 1015-1021, 1992

Protein p67: a calcium-binding protein localized at the sarcolemma of secretary atrial myocytes.

Iida H, Nishitani H, Shibata Y

Circ. Res. 70: 370-381, 1992

RCC1 is a nuclear protein required for coupling activation of cdc2 kinase with DNA synthesis and for start of the cell cycle.

Seino H, Nishitani H, Seki T, Hisamoto N, Tazunoki T, Shiraki N, Ohtsubo M,Yamashita K, Sekiguchi T, Nishimoto T

Cold Spring Harbor Symposia on Quantitative Biology LVI: 369-375, 1991

Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis.

Nishitani H, Ohtsubo M, Yamashita K, Iida H, Pines J, Yasuda H, Shibata Y, Nishimoto T

EMBO J. 10: 1555-1564, 1991

Mutation of hamster cell cycle gene RCC1 is complemented by the homologous gene of Drosophila and S. cerevisiae.

Ohtsubo M, Yoshid T, Seino H, Nishitani H, Clark K, Sprague G, Frashch M, Nishimoto T

EMBO J. 10: 1265-1273, 1991

Okadaic acid, a potent inhibitor of type 1 and type 2A protein phosphatase, activates cdc2/H1 kinase and transiently induces a premature mitosis like state in BHK cells.

Yamashita K, Yasuda H, Pines J, Yamamoto K, Nishitani H, Ohtsubo M, Hunter T, Sugimoto T, Nishimoto T

EMBO J. 9: 4331-4338, 1990

Cloning of Xenopus RCC1 cDNA, a homologue of the human RCC1 gene; complementation and identification of the product.

Nishitani H, Kobayashi H, Ohtsubo M, Nishimoto T

10. J.Biochem. 107: 228- 235, 1990
    

Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene.

Uchida S, Sekiguchi T, Nishitani H, Miyauchi K,.Ohtsubo M, Nishimoto T

Mol. Cell. Biol. 10: 577-584, 1990

Molecular cloning of human genes that complement the temperature sensitive cell cycle mutants of BHKcells.

Nishimoto T, Kai R, Sekiguchi T, Ohtsubo M, Sasaki H, Furuno N, Uchida S, Miyauchi K, Nishitani H

Cell Cycle Control in Eukaryote, Cold Spring Harbor Laboratory.169-175, 1988

Replication intermediate of a hybrid plasmid carrying the replication terminus (ter) site of R6K as revealed byagarose gel electrophoresis.

Horiuchi T, Akiyama M, Hidaka M, Nishitani H, Sekiguchi M

Mol. Gen. Genet. 210: 394-398, 1987

Expression of Licensing factors Cdt1 and Geminin in human colon Cancer.

Bravou V, Nishitani H, Song SY,Taraviras S, Varakis J

Int. J. Oncol. 27(6): 1511-1518, 2005

DNA replication licensing.

Nishitani H, Lygerou Z

Frontiers in Bioscience 9: 2115-2132, 2004

Overexpression of the Replication Licensing Regulators hCdt1 and hCdc6  Characterizes a Subset of Non-Small-Cell Lung Carcinomas: Synergistic Effect with Mutant p53 on Tumor Growth and Chromosomal Instability--Evidence of E2F-1 Transcriptional Control over hCdt1.

Karakaidos P, Taraviras S, Vassiliou LV, Zacharatos P, Kastrinakis NG, Kougiou D, Kouloukoussa M, Nishitani H, Papavassiliou AG, Lygerou Z, Gorgoulis VG

Am. J. Pathol. 165 (4): 1351-13365, 2004

S. pombe RanGAP Homolog, SpRna1, Is Required for Centromeric Silencing and Chromosome Segregation.

Kusano A, Yoshioka T, Nishijima H, Nishitani H, Nishimoto T

Mol. Biol. Cell 15(11):4960-4970, 2004

Cdt1 and Geminin are down-regulated upon cell cycle exit and are over- expressed in cancer-derived cell lines.

Xouri G, Lygerou Z, Nishitani H, Pachnis V, Nurse P, Taraviras S

Eur. J. Biochem. 271:3368-3378, 2004

Proteolysis of DNA replication licensing factor Cdt1 in S-phase is performed independently of Geminin through its N-terminal region.

Nishitani H, Lygerou Z, Nishimoto T

J Biol. Chem. 279:30807-30816, 2004

Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding.

Sugimoto N, Tatsumi Y, Tsurumi T, Matsukage A, Kiyono T, Nishitani H, Fujita M

J Biol. Chem. 279 : 19691-19697, 2004

The reconstituted human Chl12-RFC complex functions as a second PCNA loader.

Shiomi Y, Shinozaki A, Sugimoto K, Usukura J, Obuse C, Tsurimoto T.

Genes Cells. Apr;9(4):279-790, 2004

Two ubiquitin-conjugating enzymes UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.

Seino H, Kishi T, Nishitani H, Yamao F

Mol. Cell. Biol. 23(10): 3497-3505, 2003

Caffeine mimics adenine and 2'-deoxyadenosine, both of which inhibit the guanine- nucleotide exchange activity of RCC1 and the kinase activity of ATR.

Nishijima H, Nishitani H, Saito N, Nishimoto T

Genes Cells 8(5): 423-435, 2003

Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast.

Takahashi T, Ohara E, Nishitani H, Masukata H

EMBO J. 22: 964-974, 2003

A novel nuclear protein, Twa1, and Muskelin comprise a complex with RanBPM.

Umeda M, Nishitani H, Nishimoto T

Gene 303: 47-54, 2003

RanBPM, a nuclear protein that interacts with and regulates transcriptional activity of androgen receptor and glucocorticoid receptor.

Rao MA, Cheng H, Quayle AN, Nishitani H, Nelson CC, Rennie PS

J Biol. Chem. 277: 48020-48027, 2002

Control of DNA replication licensing in a cell cycle.

Nishitani H, Lygerou Z

Genes Cells 7: 523-534, 2002

A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein.

Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T.

J Biol Chem. 2002 Oct 25;277(43):40362-7.

The reconstituted human Chl12-RFC complex functions as a second PCNA loader.

Shiomi Y, Shinozaki A, Nakada D, Sugimoto K, Usukura J, Obuse C, Tsurimoto T.

Genes Cells. 2004 Apr;9(4):279-90.

The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase.

Nishitani H, Taraviras S, Lygerou Z, Nishimoto T

J Biol. Chem. 276: 44905-44911, 2001

Full-sized RanBPM cDNA encodes a protein possessing a long stretch of proline and glutamine within the N-terminal region, comprising a large protein complex.

Nishitani H, Hirose E, Uchimura Y, Nakamura M, Umeda M, Nishii K, Mori N, Nishimoto T

Gene 272: 25-33, 2001

The cdt1 protein is required to license DNA for replication in fission yeast.

Nishitani H, Lygerou Z, Nishimoto T, Nurse P

Nature 404: 625-628, 2000

Premature Chromatin condensation caused by loss of RCC1

Nishijima H, Seki T, Nishitani H, Nishimoto T

Progress Cell Cycle Research 4: 145-156, 2000

ATP-dependent structural change of the eukaryotic clamp-loader protein, replication factor C.

Shiomi Y, Usukura J, Masamura Y, Takeyasu K, Nakayama Y, Obuse C, Yoshikawa H, Tsurimoto T.

Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14127-32.