Publications (since 2002)

2019

New Assay Method Based on Raman Spectroscopy for Enzymes Reacting with Gaseous Substrates

Y. K. Nakagawa, K. Nishikawa, S. Nakashima, S. Inoue, T. Ohta, T. Ogura,

Y. Shigeta, K. Fukutani, T. Yagi and Y. Higuchi

Protein Science, 28, 663-670 (2019)

 

2018

High-resolution Structure of Dishevelled2-DIX Domain Y27W Mutant

K. Yamanishi, Y. Sin, S. Terawaki, Y. Higuchi and N. Shibata

Acta Crystallogr., F75, 116-122 (2018)

 

Structural Changes in the Trinuclear Copper Center upon the Reduction of Bilirubin Oxidase (BOD)

T. Tokiwa, M. Shoji,V. Sladek, N. Shibata, Y. Higuchi, K. Kataoka, Y. Shigeta and F Misaizu

Molecules, 24, 76, (2018)

 

Redox-dependent Conformational Changes of a Proximal [4Fe-4S] Cluster in Hyb-type [NiFe]-hydrogenase to Protect the Active Site from O2

N. D. M. Noor, H. Matsuura, K. Nishikawa, H. Tai, S. Hirota, J. Kim, J. Kang,

M. Tateno, K-S. Yoon, S. Ogo, S. Kubota, Y. Shomura and Y. Higuchi

Chem. Commun., 54, 12385-12388 (2018)

 

Redox Potential-dependent Formation of an Unusual His–Trp Bond in Bilirubin Oxidase

M. Akter, T. Tokiwa, M. Shoji, K. Nishikawa, Y. Shigeta, T Sakurai, Y. Higuchi,

K. Kataoka and N. Shibata

Chemistry - A European Journal, 24 1-8 (2018)

 

Complete Genome Sequence of a Moderately Thermophilic, Facultative Chemolithoautotrophic Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus TH-1

H. Arai, Y. Shomura, Y. Higuchi and M. Ishii

Microbiology Resource Announcements, 7(6), e00857-18 (2018)

 

Direct Participation of a Peripheral Side Chain of a Corrin Ring in Coenzyme B12 Catalysis.

N. Shibata, Y. Sueyoshi, Y. Higuchi, and T. Toraya

Angew. Chem. Int. Ed., 57(26) 7830-7835 (2018)

 

Reactivation of standard [NiFe]-hydrogenase and bioelectrochemical catalysis of proton reduction and hydrogen oxidation in a mediated-electron-transfer system

S. Shiraiwa, K. So, Y. Sugimoto, Y. Kitazumi, O. Shirai, K. Nishikawa, Y.Higuchi and K. Kano

Bioelectrochem, 123, 156-161 (2018)

 

Construction of a Triangle-Shaped Trimer and a Tetrahedral Structure Using an 兛-Helix-Inserted Circular Permutant of Cytochrome c555

A. Oda, S. Nagao, M. Yamanaka, I. Ueda, N. Shibata, Y. Higuchi, and S. Hirota

Chem. Asian J., 13, 964-967 (2018)

 

Structural basis of correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase

S. Negoro, N.Shibata, Y.-H. Lee, I. Takehara, R. Kinugasa, K. Nagai, Y. Tanaka, D. Kato, M. Takeo, Y. Goto and Y. Higuchi

Scientific Reports, 8:9725 (2018)

 

Comprehensive reaction mechanisms at and nearby the Ni‒Fe active sites of [NiFe] hydrogenases

H. Tai, Y. Higuchi and S. Hirota

Dalton Transactions, 47, 4408-4423 (2018)

 

2017

Structural Basis of the Redox Switches in the NAD+-reducing Soluble [NiFe]-hydrogenase

Y. Shomura, M. Taketa, H. Nakashima, H. Tai, H. Nakagawa, Y. Ikeda, M. Ishii,

Y. Igarashi, H. Nishihara, K-S. Yoon, S. Ogo, S. Hirota, Y. Higuchi

Science 357, 928-932 (2017)

 

Ni-elimination from the Active Site of the Standard [NiFe]-hydrogenase upon Oxidation by O2

K. Nishikawa, S. Mochida, T. Hiromoto, N. Shibata, and Y. Higuchi

J. Inorg. Biochem., 177, 435-437 (2017)

 

Equilibrium between Inactive Ready Ni-SIr and Active Ni-SIa States of [NiFe] Hydrogenase Studied by Utilizing Ni-SIr-to-Ni-SIa Photoactivation

H. Tai, L. Xu, K. Nishikawa, Y. Higuchi and S. Hirota

Chem. Commun. 53, 10444-10447 (2017)

 

Formation and Carbon Monoxidedependent Dissociation of Allochromatium vinosum Cytochrome cOligomers Using Domain-swapped Dimers

M. Yamanaka, M. Hoshizumi, S. Nagao, R. Nakayama, N. Shibata Y. Higuchi and S. Hirota

Protein Science, 26(3), 464-474 (2017)

 

Electrostatic Roles in Electron Transfer from [NiFe] hydrogenase to Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F

Y. Sugimoto, Y.  Kitazumi, O. Shirai, K. Nishikawa, Y. Higuchi, M. Yamamoto and K. Kano

Biochim. Biophys. Acta, 1865, 481-487 (2017)

 

Rational Design of Domain-Swapping-Based c-Type Cytochrome Heterodimers by Using Chimeric Proteins

M. Zhang, T. Nakanishi, M. Yamanaka, S. Nagao, S. Yanagisawa, Y. Shomura, N. Shibata, T. Ogura, Y. Higuchi, and S. Hirota

ChemBioChem, 18, 15 (2017)

 

Structural Basis for Ccd1 Autoinhibition in the Wnt Pathway through Homomerization of the DIX Domain

S. Terawaki, S. Fujita, T. Katsutani, K. Shiomi, K. Keino-Masu, M. Masu, K. Wakamatsu, N. Shibata and Y. Higuchi

Scientific Report, 7(1), 7739 (2017), July, DOI: 10.1038/s41598-017-08019-5

 

2016

Domain Swapping Oligomerization of Thermostable c-type Cytochrome in E. coli Cells

Y. Hayashi, M. Yamanaka, S. Nagao, H. Komori, Y. Higuchi and S. Hirota

Scientific Report Feb 3;6:19334. (2016)

 

Synthesis and Reactivity of a Water-soluble NiRu Monohydride Complex with a Tethered Pyridine Moiety

T. Matsumoto, K. Yoshimoto, C. Zheng, Y. Shomura, Y. Higuchi, H. Nakai and S. Ogo

Chem. Lett. 45, 197–199 (2016)

 

Improved Purification, Crystallization and Crystallographic Study of Hyd-2 Type [NiFe]-hydrogenase from Citrobacter sp. S-77.

N. D. M. Noor, K Nishikawa, H. Nishihara, K-S. Yoon, S. Ogo and Y. Higuchi

Acta Crystallogr. F72 (1), 53-58 (2016)

 

Bioelectrochemical analysis of thermodynamics of the catalytic cycle and kinetics of the oxidative inactivation of oxygen-tolerant [NiFe]-hydrogenase.

K. So, R. Hamamoto, R. Takeuchi, Y. Kitazumi, O. Shirai, R. Endo, H. Nishihara, Y. Higuchi, K. Kano

J. Electroanal. Chem. 766, 152-161 (2016)

 

Crystal Structure of a Hypothetical Protein, TTHA0829 from Thermus thermophilus HB8, Composed of Cystathionine--synthase (CBS) and Aspartate-kinase Chorismate-mutase tyrA (ACT) Domains

M. Nakabayashi, N. Shibata, E. Ishido-Nakai, M. Kanagawa, Y. Iio, H. Komori, Y. Ueda, N. Nakagawa, S. Kuramitsu and Y. Higuchi

Extremophiles, 20 (3), 275-282 (2016)

 

Direct Electron Transfer-Type Dual Gas Diffusion H2/O2 Biofuel Cells

K. So, Y. Kitazumi, O. Shirai, K. Nishikawa, Y. Higuchi and K. Kano

J. Mat. Chem., 4, 8742-8749 (2016)

 

Exogenous Acetate Ion Reaches the Type II Copper Centre in CueO Through the Water-Excretion Channel and Potentially Affects the Enzymatic Activity

H. Komori, K. Kataoka, S. Tanaka, N. Matsuda, Y. Higuchi and T. Sakurai

Acta Crystallogr., F72, 558-563 (2016)

 

Photoactivation of the Ni-SIr State to the Ni-SIa State in [NiFe] Hydrogenase: FT-IR Study on the Light Reactivity of the Ready Ni-SIr State and As-isolated Enzyme Revisited

H. Tai, L. Xu, S. Inoue, K. Nishikawa, Y. Higuchi and S. Hirota

Phys. Chem. Chem. Phys, 18 22025-22030 (2016)

 

Biochemical, Spectroscopic and X-ray Structural Analysis of Deuterated Multicopper Oxidase CueO Prepared from a New Expression Construct for Neutron Crystallography

M. Akter, C. Inoue, H. Komori, N. Matsuda, T. Sakurai, K. Kataoka, Y. Higuchi and N. Shibata

Acta Crystallogr., F72, 788-794 (2016)

 

Dual Gas-diffusion Membrane- and Mediatorless Dihydrogen/Airbreathing Biofuel Cell Operating at Room Temperature

H. Xia, K. So, Y. Kitazumi, O. Shirai, K. Nishikawa, Y. Higuchi and K. Kano

Journal of Power Sources, 335, 105-112 (2016)

 

Structure and Function of [NiFe] Hydrogenases

H. Ogata, W. Lubitz and Y. Higuchi

J. Biochem. (Tokyo), 160(5), 251-258 (2016)

 

Mutations Affecting the Internal Equilibrium of the Reaction Catalyzed by 6-aminohexanoate-dimer Hydrolase

S. Negoro, Y. Kawashima, N. Shibata, T. Kobayashi, T. Baba, Y-H. Lee, K. Kamiya, Y. Shigeta, K. Nagai, I. Takehara, D. Kato, M. Takeo, and Y. Higuchi

FEBS Lett., 590(18), 3133-3143 (2016)

 

2015

Domain-swapped Dimer of Pseudomonas aeruginosa Cytochrome c551: Structural Insights into Domain Swapping of Cytochrome c Family Proteins

S. Nagao, M. Ueda, H. Osuka, H. Komori, H. Kamikubo, M. Kataoka, Y. Higuchi and S. Hirota

PLoS One, 10(4): e0123653 (2015)

 

Structural Basis for Cargo Binding and Autoinhibition of Bicaudal-D1 by a Parallel Coiled-coil with Homotypic Registry

S. Terawaki, A. Yoshikan, Y. Higuchi, K. Wakamatsu

Biochem. Biophys. Res. Commun. 460(2), 451-456 (2015)

 

FT-IR Characterization of the Light-Induced Ni-L2 and Ni-L3 States of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F

H. Tai, K. Nishikawa, S. Inoue, Y. Higuchi and S. Hirota

J. Phys. Chem. B 119 (43), 13668–13674 (2015)

 

Crystallization and Preliminary X-ray Diffraction Analysis of FAD-dependent Glucose Dehydrogenase

H. Komori, K. Inaka, N. Furubayashi, M. Honda and Y. Higuchi

Acta Crystallogr. F71, 1017-1019 (2015)

 

"Newton's Cradle" Proton Relay with Amide-imidic Acid Tautomerization in Inverting Cellulase Visualized by Neutron Crystallography

A. Nakamura, T. Ishida, K. Kusaka, T. Yamada, S. Fushinobu, I. Tanaka, S. Kaneko, K. Ohta, H. Tanaka, K. Inaka, Y. Higuchi, N. Niimura, M. Samejima and K. Igarashi

Science Advances, Aug; 1(7): e1500263 (2015)

 

Nanoscale Charge Transport in Cytochrome c3/DNA Network: Comparative Studies between Redox-active Molecules

H. Yamaguchi, D.-C. Che, Y. Hirano, M. Suzuki, Y. Higuchi, and T. Matsumoto

Jap. J. of App. Physics 54, 095201-(1-4) (2015)

 

Molecular architectures and functions of radical enzymes and their (re)activating proteins.

N. Shibata and T. Toraya

J. Biochem. (Tokyo) 158(4), 271-292 (2015)

 

Structural Insights into the O2 Reduction Mechanism of Multicopper Oxidase
H. Komori and Y.Higuchi
 
J. Biochem. (Tokyo) 158(4), 293-298 (2015)

 

Oligomerization Enhancement and Two Domain Swapping Mode Detection for Thermostable Cytochrome c-552 via the Elongation of the Major Hinge Loop

C. Ren, S. Nagao, M. Yamanaka, H. Komori, Y. Shomura, Y. Higuchi and S. Hirota

Mol. Biosyst. 11(12), 218-221 (2015)

 

Domain-swapped Cytochrome cb562 Dimer and Its Nanocage Encapsulating a Zn-SO4 Cluster in the Internal Cavity

T. Miyamoto, M. Kuribayashi, S. Nagao, Y. Shomura, Y. Higuchi, and S. Hirota

Chem. Sci. 6, 7336-7342 (2015)

 

Hydrogens Detected by Subatomic Resolution Protein Crystallography in a [NiFe] hydrogenase

H. Ogata, K. Nishikawa and W. Lubitz

Nature, 520, 571-574 (2015)

 

Crystallization and Preliminary X-ray Analysis of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1

M. Taketa, H. Nakagawa, M. Habukawa, H. Osuka, K. Kihira, H. Komori, N. Shibata, M. Ishii, Y. Igarashi, H. Nishihara, K-S. Yoon, S. Ogo, Y. Shomura and Y. Higuchi

Acta Crystallogr. F71, 96–99 (2015).

 

Change in Structure and Ligand Binding Properties of Hyperstable Cytochrome c555 from Aquifex aeolicus by Domain Swapping

M. Yamanaka, S. Nagao, H. Komori, Y. Higuchi and S. Hirota

Protein Science, 24, 366-375 (2015)

 

Refined Regio- and Stereoselective Hydroxylation of L-Pipecolic Acid by the Engineered L-Proline-cis-4-Hydroxylase

K. Koketsu, K. Moriwaki, Y. Shomura, M. Hayashi, S. Mitsuhashi, R. Hara, K. Kino and Y. Higuchi

Synthetic Biology 4 (4), 383-392 (2015)

 

2014

Rational Design of Heterodimeric Protein Using Domain Swapping for Myoglobin

Y.-W. Lin, S. Nagao, M. Zhang, Y. Shomura, Y. Higuchi, and S. Hirota

Angew. Chem. Int. Ed. 54, 511-515 (2014).

 

Control of Transition between Ni-C and Ni-SIa States in Catalytic Cycle of [NiFe] Hydrogenase by Redox State of its Proximal Fe-S Cluster

H. Tai, K. Nishikawa, M. Suzuki, Y. Higuchi, and S. Hirota

Angew. Chem. Int. Ed. 53, 13817-13820 (2014).

 

Gas-diffusion and Direct Electron Transfer-type Bioanode for Hydrogen Oxidation with Oxygen-tolerant [NiFe]-hydrogenase as an Electrocatalyst

K. So, Y. Kitazumi, O. Shirai, K. Kurita, H. Nishihara, Y. Higuchi, K. Kano

Chemistry Lett. 43, 1575-1577 (2014).

 

Kinetic Analysis of Inactivation and Enzyme Reaction of Oxygen-tolerant [NiFe]-hydrogenase at Direct Electron Transfer-type Bioanode

K. So, Y. Kitazumi, O. Shirai, K. Kurita, H. Nishihara, Y. Higuchi, and K. Kano

Bull. Chem. Soc. Jpn. 87, 1177–1185 (2014).

 

New Insights into the Catalytic Active Site Structure of Multicopper Oxidase

H. Komori, R. Sugiyama, K. Kataoka, K. Miyazaki, Y. Higuchi and T. Sakurai

Acta crysallogr. D70, 772-779 (2014).

 

Catalytic Cycle of Cytochrome-c3 Hydrogenase, a [NiFe]-enzyme, Deduced from the Structures of the Enzyme and the Enzyme Mimic.

T. Yagi, S. Ogo and Y. Higuchi

Int. J. Hydrogen. Energy 39 (32), 18543-18550 (2014).

 

Crystallographic characterization of C-terminal coiled-coil region of mouse Bicaudal-D1 (BICD1)

S. Terawaki, H. Ootsuka, Y. Higuchi and K. Wakamatsu

Acta Crystallogr. F70, 1103-116 (2014).

 

Light-Driven Hydrogen Production by Hydrogenases and a Ru-Complex inside a Nanoporous Glass Plate under Aerobic External Conditions

T. Noji, M. Kondo, T. Jin, T. Yazawa, H. Osuka, Y. Higuchi, M. Nango, S. Itoh and T. Dewa

J. Phys. Chem. Lett. 5 (14), 2402-2407 (2014).

 

Formation of Domain-Swapped Oligomer of Cytochrome c from Its Molten Globule State Oligomer

M. S. Deshpande, P. P. Parui, H. Kamikubo, M. Yamanaka, S. Nagao, H. Komori, M. Kataoka, Y. Higuchi, and S. Hirota

Biochemistry 53 (28), 4696-703 (2014).

 

2013

Photosensitivity of the Ni-A State of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F with Visible Light

H. Osuka, Y. Shomura, H. Komori, N. Shibata, S. Nagao, Y. Higuchi, S. Hirota

Biochem. Byophys. Res. Commun. 430, 284-288, (2013)

 

Studies on Hydrogenase

T. Yagi and Y. Higuchi

Proc. Jpn. Acad. Ser. B 89, 16-33 (2013)

 

Crystal Structure of the CueO Mutants at Glu506, the Key Amino Acid Located in the Proton Transfer Pathway for Dioxygen Reduction

H. Komori, T. Kajikawa, K. Kataoka, Y. Higuchi and T. Sakurai

Biochem. Biophys. Res. Commun. 438, 686-690 (2013)

 

Crystallization and X-ray Diffraction Analysis of Nylon Hydrolase (NylC) from Arthrobacter sp. KI72.

K. Nagai, K. Yasuhira, Y. Tanaka, D. Kato, M. Takeo, Y. Higuchi, S. Negoro andN. Shibata

Acta Cryst. F 69, 1151-1154 (2013).

 

Structural Aspects of [NiFe]-hydrogenases.

Y. Shomura, Y. Higuchi

Reviews in Inorganic Chemistry 33, 173-192 (2013).

 

Formation of Oligomeric Cytochrome c during Folding by Intermolecular Hydrophobic Interaction between N- and C-Terminal 兛-Helices

P. P. Parui, M. S. Deshpande, S. Nagao, H. Kamikubo., H. Komori, Y. Higuchi, M. Kataoka and S. Hirota

Biochemistry 52(48), 8732-8744 (2013).

 

Complexes of Ferriheme Nitrophorin 4 with Low-molecular Weight Thiol(ate)s Occurring in Blood Plasma

C. He, K. Nishikawa, Ö. F. Erdem, E. Reijerse, H. Ogata, W. Lubitz, M. Knipp

J. Inorg. Biochem. 122, 38-48 (2013)

 

Phase-diagram-guided method for growth of a large crystal of glycoside hydrolase family 45 inverting cellulase suitable for neutron structural analysis

A. Nakamura, T. Ishida, S. Fushinobu, K. Kusaka, I. Tanaka, K. Inaka, Y. Higuchi, M. Masaki, K. Ohta, S. Kaneko, N. Niimura, K. Igarashi and M. Samajima

J. Synchrotron Rad. 20, 859-863 (2013).

 

Inhibitory Activity of Filipendula ulmaria Constituents on Recombinant Human Histidine Decarboxylase

Y. Nitta, H. Kikuzaki, T. Azuma, Y. Ye, M. Sakaue, Y. Higuchi, H. Komori,

H. Ueno

Food. Chem. 138, 1551-1556, (2013)

 

2012

The Three-dimensional Structure of Nylon Hydrolase and the Mechanism of nylon-6 Hydrolysis

S. Negoro1, N. Shibata., Y. Tanaka ,K. Yasuhira, H. Shibata, H. Hashimoto, Y.-H. Lee, S. Oshima, R. Santa, S. Oshima, K. Mochiji, Y. Goto, T. Ikegami, K. Nagai, D. Kato, M. Takeo and Y. Higuchi

J. Biol. Chem., 287, 5079-5090 (2012).

 

An O-Centered Structure of the Trinuclear Copper Center in the Cys500Ser/Glu506Gln Mutant of CueO and Structural Changes in Low to High X-Ray Dose Conditions

H. Komori, R. Sugiyama, K. Kataoka, Y. Higuchi and T. Sakurai

Angew. Chem. Int. Ed., 51(8), 1861-1864 (2012).

 

Crystal Structure Analysis of the Translation Factor RF3 (release factor 3).

K. Kihira, Y. Shimizu, Y. Shomura, N. Shibata, M. Kitamura, A. Nakagawa, T. Ueda,K. Ochi, Y.Higuchi

FEBS Letters 586, 3705-3709 (2012).

 

Purification, Structural Basis for the Reaction Mechanism of S-carbamoylation of HypE by HypF in the Maturation of [NiFe]-hydrogenases.

Y. Shomura, Y. Higuchi

J. Biol. Chem. 287, 28409-28419 (2012).

 

Structural and Enzymatic Characterization of BacD, An l-amino Acid Dipeptide Ligase from Bacillus subtilis.

Y. Shomura, E. Hinokuchi, H. Ikeda, A. Senoo, Y. Takahashi, J. Saito, H. Komori, N. Shibata, Y. Yonetani and Y. Higuchi

Protein Science 21, 707-716 (2012).

 

Domain Swapping of the Heme and N-Terminal -Helix in Hydrogenobacter thermophilus Cytochrome c552 Dimer.

Y. Hayashi, S. Nagao, H. Osuka, H. Komori, Y. Higuchi, S. Hirota,

Biochemistry 51, 8608-8616 (2012).

 

Structural and Oxygen Binding Properties of Dimeric Horse Myoglobin.

S. Nagao, H. Osuka, T. Yamada, T. Uni, Y. Shomura, K. Imai, Y. Higuchi, S. Hirota

Dalton Transactions 41, 11378-11385 (2012).

 

Structural Study Reveals Ser345 Determines Substrate Specificity on Human Histidine Decarboxylase.

H. Komori, Y. Nitta, H. Ueno, Y. Higuchi

J. Biol. Chem. 287, 29175-29183 (2012).

 

Purification, Crystallization, and Preliminary X-ray Analysis of Human Histidine Decarboxylase.

H. Komori, Y. Nitta, H. Ueno, Y. Higuchi

Acta Crystallogr F68, 675-677 (2012).

 

2011

Structural Basis for a [4Fe-3S] Cluster in the Oxygen-tolerant Membrane-bound
 [NiFe]-hydrogenase
.

Y. Shomura, K. Yoon, H. Nishihara and Y. Higuchi

Nature 479, 253-257 (2011).

 

Crystallographic Characterization of the DIX Domain of the Wnt Signalling Positive Regulator Ccd1.

S. Terawaki, K. Yano, T. Katsutani, K. Shiomi, K. Keino-Masu, M. Masu, Y. Shomura, H. Komori, N. Shibata and Y. Higuchi,

Acta Crystallogr. F67, 758-761 (2011).

 

Crystallization and Preliminary X-ray Diffraction Analysis of Membrane-bound Respiratory [NiFe] Hydrogenase from Hydrogenovibrio marinus.

Y. Shomura, K. Hagiya, K. Yoon, H. Nishihara and Y. Higuchi

Acta Crystallogr. F67, 827-829 (2011).

 

Crystallization and X-ray diffraction analysis of nylon oligomer hydrolase (NylC) from Agromyces sp. KY5R.

K. Yasuhira, N. Shibata, Y. Tanaka, N. Kumagai, Y. Tanaka, K. Nagai, D. Kato, M. Takeo, S. Negoro and Y. Higuchi

Acta Crystallogr. F67, 892-895 (2011).

 

Role of p-Electron Systems in Stabilization of the Oxidized Tetraheme Architecture in Cytochrome c3

Y. Takayama,. M. Taketa-Sato, H. Komori, K. Morita, Su-Jin Kang, Y. Higuchi and H. Akutsu

Bull. Chem. Soc. Jpn. Vol. 84, No. 10, 1096-1101 (2011).

 

Molecular Dynamics Studies on Mutational Structures of a Nylon-6 Byproduct-degrading Enzyme.

T. Baba, K. Kamiya, T. Matsui, N. Shibata, Y. Higuchi, T. Kobayashi, S. Negoro and Y. Shigeta

Chem. Phys. Lett. 507, 157-161 (2011).

 

How Coenzyme B12-dependent Ethanolamine Ammonialyase Deals with Both Enantiomers of 2-Amino-1-propanol as Substrates: Structure-based Rationalization.

N. Shibata, Y. Higuchi and T. Toraya

Biochemistry 50, 591-598 (2011).

 

A Functional Role of the Glycosylated N-terminal Domain of Chondromodulin-I.

J. Kondo, H. Shibata, S. Miura, A. Yamakawa, K. Sato, Y. Higuchi, C. Shukunami and Y. Hiraki

J. Bone Miner. Metab. 29, 23-30 (2011).

 

Positively Charged Domain Located Downstream of PIP Box, Together with TD Amino Acids within PIP Box, is Important for CRL4Cdt2-mediated Proteolysis.

M. Michishita, A. Morimoto, T. Ishii, H. Komori, Y. Shiomi, Y. Higuchi and H. Nishitani

Genes to Cells 16, 12-22 (2011).

 

2010

Purification, Crystallization and Preliminary X-ray Analysis of the Dissimilatory Sulfite Reductase from Desulfovibrio vulgaris Miyazaki F.

H. Ogata, Y. Shomura, A.G. Agrawal, A.P. Kaur, W. Gärtner, Y. Higuchi and W. Lubitz,

Acta Crystallogr. F66, 1470-1472 (2010).

 

Crystallization and Preliminary X-ray Analysis of Dimeric and Trimeric Cytochromes c from Horse Heart.

M. Taketa, H. Komori, Y. Hattori, S. Nagao, S. Hirota and Y. Higuchi

Acta Crystallogr. F66, 1477-1479 (2010).

 

Crystal Structure Analysis of Bacillus subtilis Ferredoxin-NADP+ Oxidoreductase and the Structural Basis for Its Substrate Selectivity.

H. Komori, D. Seo, T. Sakurai and Y. Higuchi

Protein Science 19(12), 2279-2290 (2010).

 

Cytochrome c Polymerization by Successive Domain Swapping at the C-terminal helix

S. Hirota, Y. Hattori, S. Nagao, M. Taketa, H. Komori, H. Kamikubo, Z. Wang, I. Takahashi, S. Negi, Y. Sugiura, M. Kataoka and Y. Higuchi

Pro. Natl. Acad. Sci. 107(29), 12854-12859 (2010)

 

Crystal Structures of Ethanolamine Ammonia-lyase complexed with Coenzyme B12 Analogs and Substrates.

N. Shibata, H. Tamagaki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, K. Mori, N. Yasuoka, Y. Higuchi and T. Toraya

J. Biol. Chem. 285(34), 26484-26493 (2010).

 

Expression, Crystallization and Preliminary X-ray Crystallographic Study of Ethanolamine Ammonia-lyase from Escherichia coli.

N. Shibata, H. Tamagaki, S. Ohtsuki, N. Hieda, K. Akita, H. Komori, Y. Shomura,

S. Terawaki, T. Toraya, N. Yasuoka and Y. Higuchi

Acta Crystallogr. F66, 709-711 (2010).

 

Structure and Molecular Evolution of Multicopper Blue Proteins.

H. Komori and Y. Higuchi

BIOMOLECULAR CONCEPTS 1, 31-40 (2010).

 

Enzymatic Synthesis of Nylon-6 units in Organic Solvent Contained Low-water: Structural Requirement of 6-aminohexanoate-dimer Hydrolase for Efficient Amide Synthesis.

Y. Kawashima, K. Yasuhira, N. Shibata,Y Matsuura, Y. Tanaka, M. Taniguchi, Y. Miyoshi, M. Takeo, D. Kato, Y. Higuchi and S. Negoro

J. Mol. Cat. B 64, 81-88 (2010).

 

Crystallization and Preliminary X-ray Studies of Ferredoxin-NADP+ Oxidoreductase Encoded by Bacillus subtilis yumC.

H. Komori, D. Seo, T. Sakurai and Y. Higuchi

Acta Crystallogr. F66, 301-303 (2010).

 

Crystal Structure of NADH:rubredoxin Oxidoreductase from Clostridium acetobutylicum: A Key Component of the Dioxygen Scavenging System in Obligatory Anaerobes.

K. Nishikawa, Y. Shomura, S. Kawasaki, Y. Niimura and Y. Higuchi

Proteins 78(4), 1066-1070 (2010).

 

Concerto Catalysis.Harmonising [NiFe] Hydrogenase and NiRu Model Catalysts.

K. Ichikawa, K. Nonaka, T. Matsumoto,B. Kure, K-S. Yoon, Y. Higuchi, T. Yagi and S. Ogo

Dalton Transactions 39, 2993-2994 (2010).

 

Crystallization and Preliminary X-ray Analysis of NADH:rubredoxin Oxidoreductase from Clostridium acetobutylicum.

K. Nishikawa, Y. Shomura, S. Kawasaki, Y. Niimura and Y. Higuchi

Acta Crystallogr. F66, 23-25 (2010).

 

The PHCCEx Domain of Tiam1/2 is a Novel Protein- and Membrane-binding Module.

S. Terawaki, K. Kitano, T. Mori, Y. Zhai, Y. Higuchi, N. Itoh, T. Watanabe, K. Kaibuchi and T. Hakoshima

EMBO J. 29, 236-250 (2010).

 

X-ray Crystallographic Analysis of the 6-aminohexanoate Cyclic Dimer Hydrolase: Catalytic Mechanism and Evolution of an Enzyme Responsible for Nylon-6 Byproduct Degradation.

K. Yasuhira, N. Shibata, G. Mongami, Y. Uedo, Y. Atsumi, Y. Kawashima, A. Hibino, Y. Tanaka, Y-H. Lee, D. Kato, M. Takeo, Y. Higuchi and S. Negoro

J. Biol. Chem. 285, 1239-1248 (2010).

 

2009

[NiFe] Hydrogenases: Structural and Spectroscopic Studies of the Reaction Mechanism.

H. Ogata, W. Lubitz and Y. Higuchi

Dalton Transactions 37, 7577-7587 (2009).

 

Structure Analysis of the Flavoredoxin from Desulfovibrio vulgaris Miyazaki F Reveals Key Residues that Discriminate the Functions and Properties of the Flavin Reductase Family.

N. Shibata, Y. Ueda, D. Takeuchi, Y. Haruyama, S. Kojima, J. Sato, Y. Niimura, M. Kitamura and Y. Higuchi

FEBS J. 276, 4840-4853 (2009).

 

Molecular Design of Nylon-6 Byproduct Degrading Enzyme from Carboxylesterase with b-lactamase Fold.

Y. Kawashima, T. Ohki, N. Shibata, Y. Higuchi, Y. Wakitani, Y. Matsuura, Y. Nakata, M. Takeo, D. Kato and S. Negoro

FEBS J. 276, 2547-2556 (2009).

 

Two Alternative Modes for Optimizing Nylon-6 Byproduct Hydrolytic Activity from a Carboxylesterase with a 兝-lactamase Fold: X-ray Crystallographic Analysis of Directly Evolved 6-aminohexanoate-dimer Hydrolase.

T. Ohki, N. Shibata, Y. Higuchi, Y. Kawashima, M. Takeo, D. Kato and S. Negoro

Protein Science 18, 1662-1673 (2009).

 

Zwitterionic Corroles: Regioselective Nucleophilic Pyridination of a

Doubly Linked Biscorrole.

S. Hiroto, N. Aratani, N. Shibata, Y. Higuchi, T. Sasamori, N. Tokitoh, H. Shinokubo and A. Osuka

Angew. Chem. Int. Ed. 48, 2388-2390 (2009).

 

X-ray Structure of a Two-domain-type Laccase: A Missing Link in the Evolution

of multi-copper proteins.

H. Komori, K. Miyazaki and Y. Higuchi

FEBS Lett. 583, 1189-1195 (2009).

 

Crystallization and Preliminary X-ray Diffraction Analysis of a Putative Two-domain-type Laccase from a Metagenome.

H. Komori, K. Miyazaki and Y. Higuchi

Acta crystallogr. F65, 264-266 (2009).

 

2008

Strategic Roles of Axial Histidines in Structure Formation and Redox Regulation of Tetraheme Cytochrome c3.

Y. Takayama, N. D. Werbeck, H. Komori, K. Morita, K. Ozawa, Y. Higuchi and H. Akutsu

Biochemistry, 47 (36), 9405–9415 (2008).

 

pH-Dependent Isotope Exchange and Hydrogenation Catalysed by Water-soluble NiRu Complexes as Functional Models for [NiFe] hydrogenases.

B. Kure, T. Matsumoto, K. Ichikawa, S. Fukuzumi, Y. Higuchi, T. Yagi and S. Ogo

Dalton Transactions, 4747 - 4755 (2008).

 

Crystallization and Preliminary X-ray Crystallographic Study of Flavoredoxin from Desulfovibrio vulgaris Miyazaki F.

Y. Ueda, N. Shibata, D. Takeuchi, M. Kitamura and Y. Higuchi

Acta crystallogr., F 64, 851-853 (2008).

 

Crystallization and Preliminary X-ray Analysis of a Class II Release Factor RF3 from a Sulfate-reducing Bacterium.

K. Kihira, S. Numata, M. Kitamura, J. Kondo, S. Terawaki, Y. Shomura, H. Komori, N. Shibata and Y. Higuchi

Acta crystallogr., F64, 622-624 (2008).

 

Hydrogen Bonds of DsrD Protein Revealed by Neutron Crystallography.

T. Chatake, Y. Higuchi, N. Mizuno, I. Tanaka, N. Niimura and Y. Morimoto

J. Synchrotron Rad., 15, 277–280 (2008).

 

Metalation of Expanded Porphyrins: A Chemical Trigger Used To Produce Molecular Twisting and Möbius Aromaticity.

Y. Tanaka, S. Saito, S. Mori, N. Aratani, H. Shinokubo, N. Shibata, Y. Higuchi, Z. S. Yoon, K. S. Kim, S. B. Noh, J. K. Park, D. Kim and A. Osuka

Angew. Chem. Int. Ed. 47, 681-684 (2008).

 

Crystallographic Characterization of the Membrane-targeting Domains of the Rac-specific Guanine Nucleotide-exchange Factors Tiam1 and Tiam2.

S. Terawaki, K. Kitano and T. Hakoshima

Acta crystallogr. F64, 1039-1042 (2008).

 

Crystallographic Characterization of the Radixin FERM Domain Bound to the Cytoplasmic tail of Membrane-type1 Matrix Metalloproteinase (MT1-MMP).

S. Terawaki, K. Kitano, M. Aoyama and T. Hakoshima

Acta crystallogr. F64, 911-913 (2008).

 

2007

Structure and Function of the Engineered Multicopper Oxidase, CueO from Escherichia coli.

K. Kataoka, H. Komori, Y. Ueki, Y. Konno, S. Tsujimura, Y. Higuchi, K. Kano and T. Sakurai

J. Mol. Biol. 373, 141-152 (2007).

 

Crystal Structures of Hydrogenase Maturation Protein HypE in the Apo and ATP-bound Forms.

Y. Shomura, H. Komori, N. Miyabe, M. Tomiyama, N. Shibata and Y. Higuchi

J. Mol. Biol. 372, 1045-1054 (2007).

 

Nylon-oligomer Degrading Enzyme/substrate Complex: Catalytic Mechanism of 6-aminohexanoate-dimer Hydrolase

S. Negoro, T. Ohki, N. Shibata, K. Sasa, H. Hayashi, H. Nakano, K. Yasuhira, D. Kato, M. Takeo and Y. Higuchi

J. Mol. Biol. 370, 142-156 (2007).

 

The DIX Domain of Dishevelled Confers Wnt Signaling by Dynamic Polymerization.

T. Schwarz-Romond, M. Fiedler, N. Shibata, P. Jonathan G. Butler, A. Kikuchi, Y. Higuchi and M. Bienz

Nat. Struct. Mol. Biol. 14, 484-492 (2007).

 

Crystallization and Preliminary X-ray Crystallographic Studies of the Axin DIX Domain.

N. Shibata, Y. Tomimoto, T. Hanamura, R. Yamamoto, M. Ueda, Y. Ueda, N. Mizuno,  H. Ogata, H. Komori, Y. Shomura, M. Kataoka, S. Shimizu, J. Kondo, H. Yamamoto, A. Kikuchi and Y. Higuchi

Acta Crystallogr. F63, 529-531 (2007).

 

A Dinuclear Ni(mu-H)Ru Complex Derived from H2.

S. Ogo, R. Kabe, K. Uehara, B. Kure, T. Nishimura, S. C. Menon, R. Harada, S. Fukuzumi, Y. Higuchi, T. Ohhara, T. Tamada and R. Kuroki

Science 316, 585-587 (2007).

 

Determination of the Role of the Carboxyl-terminal Leucine-122 in FMN-binding Protein by Mutational and Structural Analysis.

M. Kitamura, K. Terakawa, H. Inoue, T. Hayashida, K. Suto, Y. Morimoto, N. Yasuoka, N. Shibata and Y. Higuchi

J. Biochem., 141, 459-468 (2007).

 

Crystal Structure of CO-sensing Transcription Activator CooA Bound to Exogenous Ligand Imidazole.

H. Komori, S. Inagaki, S. Yoshioka, S. Aono and Y. Higuchi

J. Mol. Biol. 367, 864-871 (2007).

 

Human DNA Replication-related Element Binding Factor (hDREF) Self-association via hATC Domain Is Necessary for Its Nuclear Accumulation and DNA Binding.

D. Yamashita, H. Komori, Y. Higuchi, T. Yamaguchi, T. Osumi and F. Hirose

J. Biol. Chem. 282, 7563-7575 (2007).

 

Molecular Basis for Specificities of Reactivating Factors For adenosylcobalamin

-dependent Diol and Glycerol Dehydratases.

H. Kajiura, K. Mori, N. Shibata and T. Toraya

FEBS J. 274, 5556-5566 (2007).

 

2006

Structure of the Cadherin-related Neuronal Receptor/Protocadherin-a First Extracellular Cadherin Domain Reveals Diversity across Cadherin Families.

H. Morishita, M. Umitsu, Y. Murata, N. Shibata, K. Udaka, Y. Higuchi, H. Akutsu, T. Yamaguchi, T. Yagi and T. Ikegami

J. Biol. Chem. 281, 33650-33663 (2006).

 

Crystallization and X-ray Diffraction Analysis of 6-aminohexanoate-cyclic-dimer Hydrolase from Arthrobacter sp. KI72.

K. Yasuhira, Y. Uedo, N. Shibata, S. Negoro, M. Takeo and Y. Higuchi

Acta Crystallogr. F62, 1209-1211 (2006).

 

High Fidelity Self-Sorting Assembling of meso-Cinchomeronimide Appended meso-meso Linked Zn(II) Diporphyrins.

T. Kamada, N. Aratani, T. Ikeda, N. Shibata, Y. Higuchi, A. Wakamiya, S. Yamaguchi, K. S. Kim, Z. S. Yoon, D. Kim and A. Osuka

J. Am. Chem. Soc. 128, 7670-7678 (2006).

 

Crystallization and Preliminary X-ray Analysis of CooA from Carboxydothermus hydrogenoformans.

H. Komori, K. Satomoto, Y. Ueda, N. Shibata, S. Inagaki, S. Yoshioka, S. Aono and Y. Higuchi

Acta Crystallogr. F62, 471-473 (2006).

 

Roles of Charged Residues in pH-dependent Redox Properties of Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F.

N. Yahata, K. Ozawa, Y. Tomimoto, K. Morita, H. Komori, H. Ogata, Y. Higuchi and H. Akutsu

Biophysics 2, 45-56 (2006).

 

Mutational Analysis of 6-aminohexanoate-dimerhydrolase: Relationship between Nylon Oligomer Hydrolytic and Esterolytic Activities.

T. Ohki, Y. Wakitani, M. Takeo, K. Yasuhira, N. Shibata, Y. Higuchi and S. Negoro

FEBS Lett. 580, 5054-5058 (2006).

 

Redox-Dependent Interaction of Cytochrome c3 with [NiFe] Hydrogenase from D. vulgaris Miyazaki F.

N. Yahata, K. Ozawa, A. Nakahara, H. Ogata, Y. Higuchi and H. Akutsu

Biochemistry 45, 1653-1662 (2006).

 

A Single-Crystal ENDOR and Density Functional Theory Study of the Oxidized States of the [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F.

M. van Gastel, M. Stein, M. Brecht, O. Schröder, F. Lendzian, R. Bittl, H. Ogata, Y. Higuchi and W. Lubitz

J. Biol. Inorg. Chem. 11, 41-51 (2006).

 

Fes1p Acts as a Nucleotide Exchange Factor for the Ribosome-associated Molecular Chaperone Ssb1p.

Z, Dragovic, Y, Shomura, N, Tzvetkov, F. U, Hartl and A. Bracher

Biol. Chem. 387, 1593-1600 (2006).

 

Molecular Chaperones of the Hsp110 Family Act as Nucleotide Exchange Factors of Hsp70s.

Z. Dragovic, S. A. Broadleyv, Y. Shomura, A. Bracher and F.U. Hartl

EMBO J. 25, 2519-2528 (2006).

 

Head Module control of Mediator Interactions.

Y. Takagi, G. Calero, H. Komori, J.A. Brown, A.H. Ehrensberger, A. Hudmon, F. Asturias and R.D. Kornberg

Mol. Cell 23, 355-364 (2006).

 

2005

X-ray Crystallographic Analysis of 6-Aminohexanoate-Dimer Hydrolase Molecular Basis for The Birth of A Nylon Oligomer-degrading Enzyme.

S. Negoro, T. Ohki, N. Shibata, N. Mizuno, Y. Wakitani, J. Tsurukame, K. Matsumoto, I. Kawamoto, M. Takeo and Y. Higuchi

J. Biol. Chem. 280, 39644-39652 (2005).

 

Release of a Damaged Cofactor from a Coenzyme B12–dependent Enzyme: X-ray Structures of Diol Dehydratase-reactivating Factor.

N. Shibata, K. Mori, N. Hieda, Y. Higuchi, M. Yamanishi and T. Toraya

Structure 13, 1745-1754 (2005).

 

Crystallization and X-ray Diffraction Analysis of 6-aminohexanoate-dimer Hydrolase from Arthrobacter sp.KI72.

T. Ohki, N. Mizuno, N. Shibata, M. Takeo, S. Negoro and Y. Higuchi

Acta Crystallogr. F61, 928-930 (2005).

 

Activation Process of [NiFe] Hydrogenase Elucidated by High-Resolution X-Ray Analyses: Conversion of the Ready to the Unready State.

H. Ogata, S. Hirota, A. Nakahara, H. Komori, N. Shibata, T. Kato, K. Kano and Y. Higuchi

Structure 13, 1635-1642 (2005).

 

Structure of a Conserved Hypothetical Protein, TTHA0849 from Thermus thermophilus HB8, at 2.4 Å Resolution: A Putative Member of the StAR-related Lipid-transfer (START) Domain Super Family.

M. Nakabayashi, N. Shibata, H. Komori, Y. Ueda, H. Iino, A. Ebihara, S. Kuramitsu and Y. Higuchi

Acta Crystallogr. F61, 1027-1031 (2005).

 

Ubiquitin Ligase Activity of Transcription Factor IIH and Transcriptional Response to DNA Damage.

Y. Takagi, C. A. Masuda, W.-H. Chang, H. Komori, D. Wang, T. Hunter, C. A. P. Joazeiro and R. D. Kornberg

Mol. Cell 18, 237-243 (2005).

 

An Engineered Chaperonin Caging a Guest Protein: Structural Insights and Potential as a Protein Expression Tool.

M. Furutani, J. Hata, Y. Shomura, K. Itami, T. Yoshida, Y. Izumoto, A. Togi, A. Ideno, T. Yasunaga, K. Miki and T. Maruyama

Protein Science 14, 341-350 (2005).

 

Regulation of Hsp70 Function by HspBP1: Structural Analysis Reveals an Alternate Mechanism for Hsp70 Nucleotide Exchange.

Y. Shomura, Z. Dragovic, H. C. Chang, N. Tzvetkov, J.C. Young, J. L. Brodsky, V. Guerriero, F. U. Hartl and A. BracherR

Mol. Cell 17, 367-379 (2005).

 

Crystal Structure of Stilbene Synthase from Arachis hypogaea.

Y. Shomura, I. Torayama, D.Y. Suh, T. Xiang, A. Kita, U. Sankawa and K. Miki Proteins 26, 803-806 (2005).

 

2004

Correlation Between the g Tensors and the Nonplanarity of Porphyrin Rings in Desulfovibrio vulgaris Miyazaki F Cytochrome c3, Studied by Single Crystal EPR.

T. Saitoh, Y. Tachibana, Y. Higuchi, H Hori and H. Akutsu

Bull. Chem. Soc. Jpn. Vol. 77 No. 2 357-363 (2004).

 

Crystallization and Preliminary X-ray study of the two inhibitor complexes of the catalytic domain of death-associated protein kinase

A. Yamakawa, H. Ogata, K. Morita, N. Shibata, N. Andou, H. Sanuki, K. Yamada, T. Hioki, T. Ishii and Y. Higuchi

Acta Crystallogr. D60, 764-766 (2004).

 

Cloning and Expression of the Enolase gene from Desulfovibrio vulgaris (Miyazaki F).

M. Kitamura, Y. Takayama, S. Kojima, K. Kohono, H. Ogata, Y. Higuchi, H. Inoue

Biochim. Biophys. Acta 1676, 172-181 (2004).

 

Crystallization and MAD Data Collection of High-Molecular Weight Cytochrome c from Desulfovibrio vulgaris Miyazaki F.

N. Shibata, K. Suto, E. Ichimura, K. Yoshimura, K. Muneo, S. Tomigami, Y. Morimoto, M. Ogata, T. Yagi, Y. Higuchi and N. Yasuoka

Prot. Pep. Let. 11, No.1, 93-96 (2004).

 

X-ray Induced Reduction of the Crystal of High-Molecular Weight Cytochrome c Revealed by Microspectrophotometory.

M. Sato, N. Shibata, Y. Morimoto, Y. Takayama, K. Ozawa, H. Akutsu, Y. Higuchi and N. Yasuoka

J. Synchrotron Rad. 11, 113-116 (2004).

 

Hydration Structures in Proteins and Neutron Diffraction Experiment on Dissimilatory Sulfite Reductase D (DsrD).

T. Chatake, A. Ostermann, K. Kurihara, F. G. Parak, N. Mizuno, G. Voordouw, Y. Higuchi, I. Tanaka and N. Niimura

J.  Synchrotron Rad. 11, 72-75 (2004).

 

The N-terminal Domain of the Replication Initiator Protein RepE Is a Dimerization Domain Forming a Stable Dimer.

A. NakamuraH. Komori, G. KobayashiA. KitaC. Wada and K. Miki

Biochem. Biophys. Res. Commun. 315, 10-15 (2004).

 

DNA Apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF Reconstitution and X-ray-induced FAD Reduction

R. KortH. KomoriS. AdachiK. Miki and A. Eker

Acta Crystallogr. D60, 1205-1213 (2004).

 

2003

Structural Rationalization for the Lack of Stereospecificity in Coenzyme B12-dependent Diol Dehydratase.

N Shibata, Y. Nakanishi, M. Fukuoka, M. Yamanishi, N. Yasuoka and T. Toraya.

J. Biol. Chem. 278, 22717-22725 (2003).

 

Crystallization and Preliminary Neutron Analysis of the Dissimilatory Sulfite reductase D (DsrD) Protein from the Sulfate-reducing Bacterium Desulfovibrio vulgaris.

T. Chatake, N. Mizuno, G. Voordouw, Y. Higuchi, S. Arai, I. Tanaka and N. Niimura

Acta Crystallogr. D59, 2306-2309 (2003).

 

X-ray Crystallographic Characterization and Phasing of a Fucose Specific Lectin from Aleuria aurantia.

M. Fujihashi, D. H. Peapus, E. Nakajima, T. Yamada, J. Saito, A. Kita, Y. Higuchi, A. Ando, N. Kamiya, Y. Nagata and K. Miki

Acta Crystallogr. D59, 378-380 (2003).

 

Single Crystal EPR Studies of the Reduced Active Site of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F.

S. Foerster, M. Stein, M. Brecht, H. Ogata, Y. Higuchi and W. Lubitz

J. Am. Chem. Soc. 125, 83-93 (2003).

 

Three-dimensional Crystal Structure of Dissimilatory Sulfite Reductase D (DsrD) Protein - Possible Interaction with B- and Z-DNA by Its Winged-helix Motif.

N. Mizuno, G. Voordouw, K. Miki, A Sarai and Y. Higuchi

Structure 11, 1133-1140 (2003).

 

Role of the Aromatic Ring of Tyr43 in Tetraheme Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F.

K. Ozawa, Y. Takayama, F. Yasukawa, T. Ohmura, M. A. Cusanovich, Y., Tomimoto, H Ogata, Y. Higuchi and H. Akutsu

Biophys. J. 85, 3367-3374 (2003).

 

2002

Structural Studies of the Carbon Monoxide Complex of [NiFe]hydrogenase from Desulfovibrio vulgaris Miyazaki F: Suggestion for the Initial Activation Site for Dihydrogen.

H. Ogata, Y. Mizoguchi, N. Mizuno, K. Miki, S. Adachi, N. Yasuoka, T. Yagi, O. Yamauchi, S. Hirota and Y. Higuchi

J. Am. Chem. Soc. 124, 11628-11635 (2002).

 

Substrate-induced Conformational Change of a Coenzyme B12-Dependent Enzyme: Crystal Structure of the Substrate-Free Form of Diol Dehydratase.

N. Shibata, J. Masuda, Y. Morimoto, N. Yasuoka and T. Toraya

Biochemistry 41, 12607-12617 (2002).

 

Crystal Structure of Coenzyme B12-Dependent Glycerol Dehydratase in Complex with Cobalamin and Propane-1,2-Diol.

M. Yamanishi, M. Yunoki, T. Tobimatsu, H. Sato, J. Matsui, A. Dokiya, Y. Iuchi, K. Oe, K. Suto, N. Shibata, Y. Morimoto, N. Yasuoka and T. Toraya

Eur. J. Biochem. 269, 4484-4494 (2002).

 

Crystal Structure of Activated Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from Green Alga Chlamydomonas reinhardtii Complexed with 2-Carboxyarabinitol-1,5- Bisphosphate.

E. Mizohata, H. Matsumura, Y. Okano, M. Kumei, H. Takuma, J. Onodera, K. Kato, N. Shibata, T. Inoue, A. Yokota and Y. Kai:

J. Mol. Biol. 316, 677-689 (2002).